MATRIEX imaging: multiarea two-photon real-time in vivo explorer
文献类型：期刊论文
| 作者 | M.K.Yang; Z.Q.Zhou; J.X.Zhang; S.S.Jia; T.Li; J.H.Guan |
| 刊名 | Light-Science & Applications
 |
| 出版日期 | 2019 |
| 卷号 | 8页码:11 |
| 关键词 | field-of-view,auditory-cortex,brain,microscopy,signals,Optics |
| ISSN号 | 2047-7538 |
| DOI | 10.1038/s41377-019-0219-x |
| 英文摘要 | Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions in mammalian brains. However, the extent of such studies is typically confined to a single functional region of the brain. Here, we demonstrate a novel technique, termed the multiarea two-photon real-time in vivo explorer (MATRIEX), that allows the user to target multiple functional brain regions distributed within a zone of up to 12 mm in diameter, each with a field of view (FOV) of similar to 200 mu m in diameter, thus performing two-photon Ca2+ imaging with single-cell resolution in all of the regions simultaneously. For example, we demonstrate real-time functional imaging of single-neuron activities in the primary visual cortex, primary motor cortex and hippocampal CA1 region of mice in both anesthetized and awake states. A unique advantage of the MATRIEX technique is the configuration of multiple microscopic FOVs that are distributed in three-dimensional space over macroscopic distances (>1 mm) both laterally and axially but that are imaged by a single conventional laser scanning device. In particular, the MATRIEX technique can be effectively implemented as an add-on optical module for an existing conventional single-beam-scanning two-photon microscope without requiring any modification to the microscope itself. Thus, the MATRIEX technique can be readily applied to substantially facilitate the exploration of multiarea neuronal activity in vivo for studies of brain-wide neural circuit function with single-cell resolution. |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/62876]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | M.K.Yang,Z.Q.Zhou,J.X.Zhang,et al. MATRIEX imaging: multiarea two-photon real-time in vivo explorer[J]. Light-Science & Applications,2019,8:11. |
| APA | M.K.Yang,Z.Q.Zhou,J.X.Zhang,S.S.Jia,T.Li,&J.H.Guan.(2019).MATRIEX imaging: multiarea two-photon real-time in vivo explorer.Light-Science & Applications,8,11. |
| MLA | M.K.Yang,et al."MATRIEX imaging: multiarea two-photon real-time in vivo explorer".Light-Science & Applications 8(2019):11. |
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