Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway
文献类型：期刊论文
| 作者 | M.L.Nan; X.Wang; H.J.Li; D.H.Yu; W.Y.Sun; H.M.Xu; Y.F.He |
| 刊名 | Oncology Letters
 |
| 出版日期 | 2019 |
| 卷号 | 17期号:1页码:630-637 |
| 关键词 | rotundic acid,apoptosis,p53,MCF-7 cells,breast-cancer cells,triterpene acids,caspase 3,expression,reconstitution,proteins,protects,trial,Oncology |
| ISSN号 | 1792-1074 |
| DOI | 10.3892/ol.2018.9616 |
| 英文摘要 | In the present study, the functions and mechanisms of rotundic acid (RA) underlying its induction of apoptosis in caspase-3-transfected MCF-7 human breast cancer cells (Cas3-MCF-7 cells) were investigated. RA induced apoptosis in Cas3-MCF-7 cells more efficiently compared with that in MCF-7 cells transfected with control plasmid. The results from an MTT assay demonstrated that RA effectively inhibited Cas3-MCF-7 cell viability in a dose-dependent manner and induced cell apoptosis via caspase-3 activity within 12 to 48 h. Western blotting and fluorescence-activated cell sorting demonstrated that RA initiated Cas3-MCF-7 cell apoptosis via p53 activation. The silencing of the p53 gene in the Cas3-MCF-7 cell line led to decreased RA-induced Cas3-MCF-7 cell caspase-3 activity and cell apoptosis. Collectively, the results of the present study indicate that caspase-3 serves a critical function in rotundic acid-induced apoptosis, and suggest that caspase-3 deficiency may contribute to the chemotherapy-resistance of breast cancer. Reconstitution of caspase-3 sensitizes MCF-7 breast cancer cells to chemotherapy. RA has the potential for development as a novel drug combined with reconstitution of caspase-3 gene therapy for the treatment of human breast cancer with caspase-3 deficiency. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/63124]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | M.L.Nan,X.Wang,H.J.Li,et al. Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway[J]. Oncology Letters,2019,17(1):630-637. |
| APA | M.L.Nan.,X.Wang.,H.J.Li.,D.H.Yu.,W.Y.Sun.,...&Y.F.He.(2019).Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway.Oncology Letters,17(1),630-637. |
| MLA | M.L.Nan,et al."Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway".Oncology Letters 17.1(2019):630-637. |
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