EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC
文献类型：期刊论文
| 作者 | Y.Liu; Y.H.Zhou; K.H.Huang; Y.Li; X.J.Fang; L.An; F.F.Wang |
| 刊名 | Aging-Us
 |
| 出版日期 | 2019 |
| 卷号 | 11期号:23页码:11054-11072 |
| 关键词 | triple-negative breast cancer,human epidermal growth factor receptor,chimeric antigen receptor engineered T cells,negative breast-cancer,growth-factor receptor,therapy,amplification,inhibition,expression,challenges,mutations,her2,Cell Biology,Geriatrics & Gerontology |
| ISSN号 | 1945-4589 |
| DOI | 10.18632/aging.102510 |
| 英文摘要 | Triple-negative breast cancer (TNBC) is an aggressive cancer subtype for which effective therapies are lacking. Epidermal growth factor receptor (EGFR) is overexpressed in various types of TNBC cells, and several EGFR-specific immunotherapies have been used to treat cancer patients. Chimeric antigen receptor engineered T (CAR-T) cells have also been used as cancer therapies. In this study, we generated two types of EGFR-specific CAR-modified T cells using lentiviral vectors with DNA sequences encoding the scFv regions of two anti-EGFR antibodies. The cytotoxic and antitumor effects of these CAR-modified T cells were examined in cytokine release and cytotoxicity assays in vitro and in tumor growth assays in TNBC cell line- and patient-derived xenograft mouse models. Both types of EGFR-specific CAR-T cells were activated by high-EGFR-expressing TNBC cells and specifically triggered TNBC cell lysis in vitro. Additionally, the CAR-T cells inhibited growth of cell-line- and patient-derived xenograft TNBC tumors in mice. These results suggest that EGFR-specific CAR-T cells might be a promising therapeutic strategy in patients with high-EGFR-expressing TNBC. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/63170]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Y.Liu,Y.H.Zhou,K.H.Huang,et al. EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC[J]. Aging-Us,2019,11(23):11054-11072. |
| APA | Y.Liu.,Y.H.Zhou.,K.H.Huang.,Y.Li.,X.J.Fang.,...&F.F.Wang.(2019).EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC.Aging-Us,11(23),11054-11072. |
| MLA | Y.Liu,et al."EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC".Aging-Us 11.23(2019):11054-11072. |
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