Single full-FOV reconstruction Fourier ptychographic microscopy
文献类型：期刊论文
| 作者 | Y. Zhu,M. Sun,X. Chen,H. Li,Q. Mu,D. Li and L. Xuan |
| 刊名 | Biomedical Optics Express
 |
| 出版日期 | 2020 |
| 卷号 | 11期号:12页码:7175-7182 |
| ISSN号 | 21567085 |
| DOI | 10.1364/BOE.409952 |
| 英文摘要 | Fourier ptychographic microscopy (FPM) is a recently developed computational imaging technique that has high-resolution and wide field-of-view (FOV). FPM bypasses the NA limit of the system by stitching a number of variable-illuminated measured images in Fourier space. On the basis of the wide FOV of the low NA objective, the high-resolution image with a wide FOV can be reconstructed through the phase recovery algorithm. However, the high-resolution reconstruction images are affected by the LED array point light source. The results are: (1) the intensities collected by the sample are severely declined when edge LEDs illuminate the sample; (2) the multiple reconstructions are caused by wavevectors inconsistency for the full FOV images. Here, we propose a new lighting scheme termed full FOV Fourier ptychographic microscopy (F3PM). By combining the LED array and telecentric lens, the method can provide plane waves with different angles while maintaining uniform intensity. Benefiting from the telecentric performance and f- property of the telecentric lens, the system stability is improved and the relationship between the position of LED and its illumination angle is simplified. The excellent plane wave provided by the telecentric lens guarantees the same wavevector in the full FOV, and we use this wavevector to reconstruct the full FOV during one time. The area and diameter of the single reconstruction FOV reached 14.6mm2 and 5.4 mm, respectively, and the diameter is very close to the field number (5.5 mm) of the 4 objective. Compared with the traditional FPM, we have increased the diameter of FOV in a single reconstruction by 10 times, eliminating the complicated steps of computational redundancy and image stitching. 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. |
| URL标识 | 查看原文 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/64888]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Y. Zhu,M. Sun,X. Chen,H. Li,Q. Mu,D. Li and L. Xuan. Single full-FOV reconstruction Fourier ptychographic microscopy[J]. Biomedical Optics Express,2020,11(12):7175-7182. |
| APA | Y. Zhu,M. Sun,X. Chen,H. Li,Q. Mu,D. Li and L. Xuan.(2020).Single full-FOV reconstruction Fourier ptychographic microscopy.Biomedical Optics Express,11(12),7175-7182. |
| MLA | Y. Zhu,M. Sun,X. Chen,H. Li,Q. Mu,D. Li and L. Xuan."Single full-FOV reconstruction Fourier ptychographic microscopy".Biomedical Optics Express 11.12(2020):7175-7182. |
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