Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor
文献类型：期刊论文
| 作者 | Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang |
| 刊名 | Cell Proliferation
 |
| 出版日期 | 2020 |
| 卷号 | 53期号:8页码:12 |
| ISSN号 | 0960-7722 |
| DOI | 10.1111/cpr.12858 |
| 英文摘要 | Objectives Traditional cancer therapy and regular immunotherapy are ineffective for treating triple-negative breast cancer (TNBC) patients. Recently, chimeric antigen receptor-engineered natural killer cells (CAR NK) have been applied to target several hormone receptors on different cancer cells to improve the efficacy of immunotherapy. Furthermore, epidermal growth factor receptor (EGFR) is a potential therapeutic target for TNBC. Here, we demonstrated that EGFR-specific CAR NK cells (EGFR-CAR NK cells) could be potentially used to treat patients with TNBC exhibiting enhanced EGFR expression. Materials and methods We investigated the cytotoxic effects of EGFR-CAR NK cells against TNBC cells in vitro and in vivo. The two types of EGFR-CAR NK cells were generated by transducing lentiviral vectors containing DNA sequences encoding the single-chain variable fragment (scFv) regions of the two anti-EGFR antibodies. The cytotoxic and anti-tumor effects of the two cell types were examined by performing cytokine release and cytotoxicity assays in vitro, and tumor growth assays in breast cancer cell line-derived xenograft (CLDX) and patient-derived xenograft (PDX) mouse models. Results Both EGFR-CAR NK cell types were activated by TNBC cells exhibiting upregulated EGFR expression and specifically triggered the lysis of the TNBC cells in vitro. Furthermore, the two EGFR-CAR NK cell types inhibited CLDX and PDX tumors in mice. Conclusions This study suggested that treatment with EGFR-CAR NK cells could be a promising strategy for TNBC patients. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/64945]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang. Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor[J]. Cell Proliferation,2020,53(8):12. |
| APA | Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang.(2020).Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor.Cell Proliferation,53(8),12. |
| MLA | Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang."Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor".Cell Proliferation 53.8(2020):12. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。