中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Compact type-I coil planet centrifuge for counter-current chromatography

文献类型:期刊论文

作者Yang Yi; Gu Dongyu; Liu Yongqiang; Aisa Haji Akber; Ito Yoichiro
刊名Journal of Chromatography A
出版日期2010
卷号1217期号:8页码:1313-1319
关键词Compact Type-i Coil Planet Centrifuge Counter-current Chromatography Dnp-amino Acids Revolution Radius Retention Of The Stationary Phase Resolution Retention Time
ISSN号0021-9673
DOI10.1016/j.chroma.2009.12.055
英文摘要

A compact type-I coil planet centrifuge has been developed for performing counter-current chromatography. It has a revolution radius of 10 cm and a column holder height of 5 cm compared with 37 and 50 cm in the original prototype, respectively. The reduction in the revolution radius and column length permits application of higher revolution speed and more stable balancing of the rotor which leads us to learn more about its performance and the future potential of type-I coil planet centrifuge. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), peak resolution (Rs), theoretical plate (N) and peak retention time (tR). The results of the experiment indicated that increasing the revolution speed slightly improved both the retention of the stationary phase and the peak resolution while the separation time is remarkably shortened to yield an excellent peak resolution at a revolution speed of 800 rpm. With a 12 ml capacity coiled column, DNP-DL-glu, DNP-β-ala and DNP-l-ala were resolved at Rs of 2.75 and 2.16 within 90 min at a flow rate of 0.4 ml/min. We believe that the compact type-I coil planet centrifuge has a high analytical potential.

学科主题Biochemistry & Molecular Biology ; Chemistry
WOS记录号WOS:000274953400017
公开日期2012-11-29
源URL[http://ir.xjipc.cas.cn/handle/365002/1849]  
专题新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
新疆理化技术研究所_资源化学研究室
作者单位1.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
3.NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA
推荐引用方式
GB/T 7714
Yang Yi,Gu Dongyu,Liu Yongqiang,et al. Compact type-I coil planet centrifuge for counter-current chromatography[J]. Journal of Chromatography A,2010,1217(8):1313-1319.
APA Yang Yi,Gu Dongyu,Liu Yongqiang,Aisa Haji Akber,&Ito Yoichiro.(2010).Compact type-I coil planet centrifuge for counter-current chromatography.Journal of Chromatography A,1217(8),1313-1319.
MLA Yang Yi,et al."Compact type-I coil planet centrifuge for counter-current chromatography".Journal of Chromatography A 1217.8(2010):1313-1319.

入库方式: OAI收割

来源:新疆理化技术研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。