Secondary Structure-Dominated Layer-by-Layer Growth Mode of Protein Coatings
文献类型:期刊论文
作者 | Lu, RF (Lu, Ruofei) 1 , 2; Zhang, XQ (Zhang, Xiaoqiang) 1 , 2; Cheng, XX (Cheng, Xinxiu) 1 , 2; Zan, XJ (Zan, Xingjie) 1 , 3; Geng, WJ (Geng, Wujun) 4 |
刊名 | LANGMUIR |
出版日期 | 2021 |
卷号 | 37期号:44页码:13000-13011 |
ISSN号 | 0743-7463 |
DOI | 10.1021/acs.langmuir.1c02062 |
英文摘要 | Benefiting from the luxury functions of proteins, protein coatings have been extended to various applications, including tissue engineering scaffolds, drug delivery, antimicrobials, sensing and diagnostic equipment, food packaging, etc. Fast construction of protein coatings is always interesting to materials science and significant to industrialization. Here, we report a layer-by-layer (LbL) multilayer-constructed coating of tannic acid (TA) and lysozyme (Lyz), in which the secondary conformations of Lyz dominate the growth rate of the TA/Lyz coating. As well characterized by various techniques (quartz crystal microbalance with dissipation (QCM-D), circular dichroism (CD) spectra, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM), contact angle, etc.), TA-induced conformational transition of Lyz to alpha-helices occurs at pH 8 from other secondary structures (beta-sheets, beta-turns, and random coils), which leads to the very fast growth of TA/Lyz with a number of deposited bilayers, with thicknesses of more than 90 nm for six bilayers. In contrast to the leading conformation of alpha-helices at pH 8, Lyz displayed multiple conformations (alpha-helices, beta-sheets, beta-turns, and random coils) at pH 6, which resulted in coating thicknesses of less than 30 nm for six bilayers. By the addition of NaCl, Tween 20, and urea, we further confirmed that the secondary conformations of Lyz relied greatly on the interactions between TA and Lyz and dominated the growth rate of the multilayers. We believe that these findings will help to understand the transformation of secondary conformations by TA or other polyphenols and inspire a new route to quickly build protein coatings. |
WOS记录号 | WOS:000718188900023 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/8107] |
专题 | 新疆理化技术研究所_资源化学研究室 |
作者单位 | 1.Wenzhou Med Univ, Dept Anesthesiol, Wenzhou Key Lab Perioperat Med, Affiliated Hosp 1, Wenzhou 325000, Peoples R China 2.Univ Chinese Acad Sci, Wenzhou Inst, Oujiang Lab Zhejiang Lab Regenerat Med Vis & Brai, Wenzhou 325001, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China |
推荐引用方式 GB/T 7714 | Lu, RF ,Zhang, XQ ,Cheng, XX ,et al. Secondary Structure-Dominated Layer-by-Layer Growth Mode of Protein Coatings[J]. LANGMUIR,2021,37(44):13000-13011. |
APA | Lu, RF ,Zhang, XQ ,Cheng, XX ,Zan, XJ ,&Geng, WJ .(2021).Secondary Structure-Dominated Layer-by-Layer Growth Mode of Protein Coatings.LANGMUIR,37(44),13000-13011. |
MLA | Lu, RF ,et al."Secondary Structure-Dominated Layer-by-Layer Growth Mode of Protein Coatings".LANGMUIR 37.44(2021):13000-13011. |
入库方式: OAI收割
来源:新疆理化技术研究所
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