Mechanism insights into polyhydroxyalkanoate-regulated denitrification from the perspective of pericytoplasmic nitrate reductase expression
文献类型：期刊论文
| 作者 | Wu, Boran2; Zhou, Meng2; Song, Liyan1 ; Xu, Qinqin2; Dai, Xiaohu2,3; Chai, Xiaoli2,3
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2021-02-01 |
| 卷号 | 754页码:9 |
| 关键词 | Polyhydroxyalkanoate (PHA) Denitrification Proteomics Solid biopolymer Pseudoinonas stutzeri |
| ISSN号 | 0048-9697 |
| DOI | 10.1016/j.scitotenv.2020.142083 |
| 通讯作者 | Dai, Xiaohu(daixiaohu@tongji.edu.cn) ; Chai, Xiaoli(xlchai@tongji.edu.cn) |
| 英文摘要 | For enhanced biological nutrient removal (BNR) process, the polyhydroxyalkanoate (PHA) can be used as an ecofriendly internal as well as external substrate for regulating the growth of heterotrophic denitrifiers and promoting the denitrification process for deep nitrogen removal from wastewater. However, the exact mechanisms by which PHA impacts bacterial metabolism and affects the electron transfer of denitrification remain unknown. In this study, the in-depth mechanism investigation for PHA-mediated denitrification based on the jointly applied transcliptomic, proteomic and Western Blotting techniques was performed on a model denitrifier, Pseudonionas stutzeri. Results showed that PHA dramatically fostered the growth of Pseudomonas stutzeri, resulting in improved nitrate removal efficiency from 32.8% to 45.8%. Comparison of protein expression profiles indicated that PHA promoted the expression of enzyme NapB and NapA by approximately 10.34 and 20.01 Limes, respectively, which were both in charge of reduction from nitrate to nitrite. Based on transcriptional sequencing and Tandem Mass Tags, the correlation results also showed that differential proteins and genes with the same expression trend were positively correlated (R-2 = 0.427, p-value<0.033). Western Blotting approach was further developed to confirm the up-regulated expression of target protein with the higher proportion of PHA in carbon source of the medium, which proved the reliability of proteomics results. All the findings presented here are believed to deepen the understanding of microbial mechanism about PHA-enhanced denitrification from the novel perspective of associated electron-transfer enzymatic proteins. (C) 2020 Published by Elsevier B.V. |
| 资助项目 | University of North Carolina at Chapel Hill ; Ministry of Science and Technology of P. R. China[2017ZX07202-002] ; Science and Technology Commission of Shanghai Municipality[18DZ1206505] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000593899600028 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/12618]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Dai, Xiaohu; Chai, Xiaoli |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Environm Microbiol & Ecol Res Ctr, 266 Fangzheng Ave, Chongqing 400714, Peoples R China 2.Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, 1239 Siping Rd, Shanghai 200092, Peoples R China 3.Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Boran,Zhou, Meng,Song, Liyan,et al. Mechanism insights into polyhydroxyalkanoate-regulated denitrification from the perspective of pericytoplasmic nitrate reductase expression[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,754:9. |
| APA | Wu, Boran,Zhou, Meng,Song, Liyan,Xu, Qinqin,Dai, Xiaohu,&Chai, Xiaoli.(2021).Mechanism insights into polyhydroxyalkanoate-regulated denitrification from the perspective of pericytoplasmic nitrate reductase expression.SCIENCE OF THE TOTAL ENVIRONMENT,754,9. |
| MLA | Wu, Boran,et al."Mechanism insights into polyhydroxyalkanoate-regulated denitrification from the perspective of pericytoplasmic nitrate reductase expression".SCIENCE OF THE TOTAL ENVIRONMENT 754(2021):9. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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