Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases
文献类型：期刊论文
| 作者 | Zhang, Youwen1; Chen, Xiaohan1; Yuan, Shaoqing2; Wang, Liang3,4 ; Guan, Xiyun1
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2020-11-17 |
| 卷号 | 92期号:22页码:15042-15049 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.0c03007 |
| 通讯作者 | Wang, Liang(wangliang83@cigit.ac.cn) ; Guan, Xiyun(xguan5@iit.edu) |
| 英文摘要 | Due to the limited clinical utility of individual biomarkers, there is growing recognition of the need for combining multiple biomarkers as a panel to improve the accuracy and efficacy of disease diagnosis and prognosis. The conventional method to detect multiple analyte species is to construct a sensor array, which consists of an array of individual selective probes for different species. In this work, by using cancer biomarker matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinases (ADAMs) as model analytes and functionalized nanographene oxide (nGO) as a sensing element, we developed a multiplexing fluorescence sensor in a nonarray format for simultaneous measurement of the activities of multiple proteases. The constructed nGO-based biosensor was rapid, sensitive, and selective and was also utilized for the successful profiling of ADAMs/MMPs in simulated serum samples. Furthermore, we showed that joint entropy and programming could be utilized to guide experiment design, especially in terms of the selection of a subset of proteases from the entire MMPs/ADAMs family as an appropriate biomarker panel. Our developed nGO-based multiplex sensing platform should find useful application in early cancer detection and diagnosis. |
| 资助项目 | National Institutes of Health[2R15GM110632-02] ; National Science Foundation[1708596] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000592852900026 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/12624]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Wang, Liang; Guan, Xiyun |
| 作者单位 | 1.Illinois Inst Technol, Dept Chem, Chicago, IL 60616 USA 2.Amazon, Seattle, WA 98121 USA 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Youwen,Chen, Xiaohan,Yuan, Shaoqing,et al. Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases[J]. ANALYTICAL CHEMISTRY,2020,92(22):15042-15049. |
| APA | Zhang, Youwen,Chen, Xiaohan,Yuan, Shaoqing,Wang, Liang,&Guan, Xiyun.(2020).Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases.ANALYTICAL CHEMISTRY,92(22),15042-15049. |
| MLA | Zhang, Youwen,et al."Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases".ANALYTICAL CHEMISTRY 92.22(2020):15042-15049. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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