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Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices

文献类型：期刊论文

作者Guo, Yuansen1,2,3; Chen, Tunan4,5; Wang, Shi4,5; Zhou, Xiaojie6; Zhang, Hua1,2,7; Li, Dandan1,2; Mu, Ning4,5; Tang, Mingjie1,2,3; Hu, Meidie1,2; Tang, Dongyun1,2,7
刊名ACS OMEGA
出版日期2020-11-24
卷号5期号:46页码:29698-29705
ISSN号2470-1343
DOI10.1021/acsomega.0c03285
通讯作者Tang, Yuzhao(tangyuzhao@zjlab.org.cn) ; Wang, Huabin(wanghuabin@citig.ac.cn)
英文摘要Traumatic brain injury (TBI) is a health problem of global concern because of its serious adverse effects on public health and social economy. A technique that can be used to precisely detect TBI is highly demanded. Here, we report on a synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopic imaging technique that can be exploited to identify TBI-induced injury by examining model mouse brain tissue slices. The samples were first examined by conventional histopathological techniques including hematoxylin and eosin (H&E) staining and 2,3,5-triphenyltetrazolium chloride staining and then spectroscopically imaged by SR-FTIR. SR-FTIR results show that the contents of protein and nucleic acid in the injured region are lower than their counterparts in the normal region. The injured and normal regions can be unambiguously distinguished from each other by the principle component analysis of the SR-FTIR spectral data corresponding to protein or nucleic acid. The images built from the spectral data of protein or nucleic acid clearly present the injured region of the brain tissue, which is in good agreement with the H&E staining image and optical image of the sample. Given the label-free and fingerprint features, the demonstrated method suggests potential application of SR-FTIR spectroscopic mapping for the digital and intelligent diagnosis of TBI by providing spatial and chemical information of the sample simultaneously.
资助项目National Natural Science Foundation of China[U1932132] ; National Natural Science Foundation of China[11604332] ; National Natural Science Foundation of China[U1632273] ; National Natural Science Foundation of China[U1732130] ; Central Government Supported Key Instrument Program of China[YXGYQ201700136] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0405] ; Natural Science Foundation of Chongqing[cstc2019jcyj-msxmX0654] ; Chongqing Collaborative Innovation Center for Brain Science
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000595527600008
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.138/handle/2HOD01W0/12655]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Tang, Yuzhao; Wang, Huabin
作者单位1.Chinese Acad Sci, Ctr Appl Phys, Chongqing 400714, Peoples R China
2.Chinese Acad Sci, Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Third Mil Med Univ, Southwest Hosp, Dept Neurosurg, Army Med Univ, Chongqing 400038, Peoples R China
5.Third Mil Med Univ, Key Lab Neurotrauma, Southwest Hosp, Army Med Univ, Chongqing 400038, Peoples R China
6.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Natl Facil Prot Sci Shanghai, Shanghai 201210, Peoples R China
7.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
8.Univ Alberta, Dept Chem & Mat Engn, Calgary, AB T6G 1H9, Canada
推荐引用方式
GB/T 7714		 Guo, Yuansen,Chen, Tunan,Wang, Shi,et al. Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices[J]. ACS OMEGA,2020,5(46):29698-29705.
APA Guo, Yuansen.,Chen, Tunan.,Wang, Shi.,Zhou, Xiaojie.,Zhang, Hua.,...&Wang, Huabin.(2020).Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices.ACS OMEGA,5(46),29698-29705.
MLA Guo, Yuansen,et al."Synchrotron Radiation-Based FTIR Microspectroscopic Imaging of Traumatically Injured Mouse Brain Tissue Slices".ACS OMEGA 5.46(2020):29698-29705.

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来源：重庆绿色智能技术研究院

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