Reversible photo-regulation on the folding/unfolding of telomere G-quadruplexes with solid-state nanopores
文献类型：期刊论文
| 作者 | Tang, Jing4,5; Wu, Ji4,5; Zhu, Rui2,5; Wang, Zhong4,5; Zhao, Chuanqi1,3; Tang, Peng4,5 ; Xie, Wanyi4,5; Wang, Deqiang4,5; Liang, Liyuan4,5
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| 刊名 | ANALYST
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| 出版日期 | 2021-01-21 |
| 卷号 | 146期号:2页码:655-663 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/d0an01930e |
| 通讯作者 | Wang, Deqiang(dqwang@cigit.ac.cn) ; Liang, Liyuan(liangliyuan@cigit.ac.cn) |
| 英文摘要 | The formation of G-quadruplexes (G4) in human telomere and other important biological regions inhibits the replication and transcription of DNA, thereby influencing further cell proliferation. The investigation of G4 formation and unfolding is vital for understanding their modulation in biological processes and life science. Photo regulation is a facile and sensitive approach for monitoring the structures of biomacromolecules and material surface properties. The nanopore-based technique is also prevalent for label-free single-molecule characterization with high accuracy. This study provides a combination of solid-state nanopore technology with light-switch as a platform for the modulation of human telomere G4 formation and splitting under switchable light exposure. The introduction of molecular switch, namely azobenzene moiety at different positions of the DNA sequence influences the formation and stability of G4. Three azobenzenes immobilized on each of the G-quartet plane (hTelo-3azo-p) or four azobenzenes on the same plane (hTelo-4azo-4p) of the human telomere G4 sequence realized the reversible control of G4 folding/unfolding at the temporal scale upon photo regulation, and the formation and splitting of G4 with hTelo-4azo-4p is slower and not thorough compared to that with hTelo-3azo-p due to the coplanar steric hindrance. Moreover, the G4 formation recorded with the combined nanopore and photo-responsive approach was also characterized with fluorescence, and the variation in the fluorescence intensity of the NMM and G4 complex exhibited a different tendency under reverse light irradiation due to the distinct interactions of NMM with the azobenzene-modified G4. Our study demonstrated a controllable and sensitive way for the manipulation of G4 structures, which will be inspiring for the intervention of G4-related cell senescence, cancer diagnosis and drug exploration. |
| 资助项目 | Youth Innovation Promotion Association[2017392] ; University of the Chinese Academy of Sciences |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000611287000032 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/12825]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Wang, Deqiang; Liang, Liyuan |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, Lab Chem Biol, Changchun 130022, Jilin, Peoples R China 2.Chongqing Jiaotong Univ, Chongqing 400014, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Jilin, Peoples R China 4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 5.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Jing,Wu, Ji,Zhu, Rui,et al. Reversible photo-regulation on the folding/unfolding of telomere G-quadruplexes with solid-state nanopores[J]. ANALYST,2021,146(2):655-663. |
| APA | Tang, Jing.,Wu, Ji.,Zhu, Rui.,Wang, Zhong.,Zhao, Chuanqi.,...&Liang, Liyuan.(2021).Reversible photo-regulation on the folding/unfolding of telomere G-quadruplexes with solid-state nanopores.ANALYST,146(2),655-663. |
| MLA | Tang, Jing,et al."Reversible photo-regulation on the folding/unfolding of telomere G-quadruplexes with solid-state nanopores".ANALYST 146.2(2021):655-663. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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