Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores
文献类型：期刊论文
| 作者 | Zhang, Mingkun1,2,3,4,5 ; Chen, Shenbao1,3,4; Hu, Jinrong1,3,4; Ding, Qihan1,3,4; Li, Linda1,3,4; Lu, Shouqin1,3,4; Long, Mian1,3,4
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| 刊名 | NANOSCALE
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| 出版日期 | 2021-03-28 |
| 卷号 | 13期号:12页码:14 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/d0nr07413f |
| 通讯作者 | Lu, Shouqin(lsq@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Conformational changes of proteins play a vital role in implementing their functions and revealing the underlying mechanisms in various biological processes. It is still challenging to monitor protein conformations with temporal fingerprints of current-resistance pulses in the nanopore technique. Here the low-resolution morphologies of different conformations of a typical integrin, alpha(x)beta(2), were estimated via relative blockade currents simulated from all-atom molecular dynamics (MD). Distinct conformational states of alpha(x)beta(2) were directly explained by the volume and shape identifiers. Protein modulation in ionic current was analyzed from the conductivity distribution inside the protein-blocked nanopore. Combining a discrete model with spheroidal approximation, a MD-based approach was developed to theoretically predict the volume and shape of the nanopore for sensing alpha(x)beta(2). This method was also applicable in specifying morphological identifiers of six other proteins, and the theoretical predictions are in good agreement with the experimental measurements. These results potentiated the validity of this method for the conformational identification of proteins in nanopores. |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000635775000012 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/13335]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Lu, Shouqin; Long, Mian |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 3.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab,Beijing Key Lab Engn Construct, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Mech, CAS Ctr Excellence Complex Syst Mech, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mingkun,Chen, Shenbao,Hu, Jinrong,et al. Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores[J]. NANOSCALE,2021,13(12):14. |
| APA | Zhang, Mingkun.,Chen, Shenbao.,Hu, Jinrong.,Ding, Qihan.,Li, Linda.,...&Long, Mian.(2021).Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores.NANOSCALE,13(12),14. |
| MLA | Zhang, Mingkun,et al."Mapping the morphological identifiers of distinct conformations via the protein translocation current in nanopores".NANOSCALE 13.12(2021):14. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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