New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves
文献类型：期刊论文
| 作者 | Xiao, Yan1,2 ; Cheng, Yuran1,2; He, Pan1,2; Wu, Xinghua3; Li, Zhe1,2
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2021-06-20 |
| 卷号 | 774页码:11 |
| ISSN号 | 0048-9697 |
| 关键词 | Atomic force microscopy Biomechanical properties Cyanobacteria and microalgae External layers Wavelet decomposition |
| DOI | 10.1016/j.scitotenv.2021.145680 |
| 通讯作者 | Li, Zhe(lizhe@cigit.ac.cn) |
| 英文摘要 | External layers, the outermost structures around cells, perform essential eco-physiological functions to support cyanobacteria and microalgae in aquatic environments. These layers have been recognized as adaptations to turbulence, a ubiquitous and inherent physical process occurring in the environments of most cyanobacteria and microalgae. However, the underlying biophysical mechanism of these layers is still poorly understood. Force measurements were performed directly on the external layers of eight living cyanobacterial and green algal strains in situ using atomic force microscopy (AFM). We developed a wavelet analysis method based on a multiscale decomposition of derivative force-distance curves to quantify the elastic responses of various external layers upon mechanical deformation. Such analysis has the advantages of detecting singularities and distinguishing the biomechanical contributions of each external layer. The elastic modulus of the same type of external layer follows the same statistical distribution. However, the elastic response among different types of external layers is challenged by our method, indicating the heterogeneity of the mechanical properties of inner and outer layers in multilayer strains. This discrepancy was due to the thickness and texture of each external layer, especially the chemical presence of ribose, hydroxyproline and glutamic acid. This study highlights a new way to elucidate more precise information about external layers and provides a biophysical mechanistic explanation for the functioning of the various external layers to protect cyanobacterial and microalgal cells in a turbulent environment. (c) 2021 Elsevier B.V. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[51779240] ; National Natural Science Foundation of China[51979262] ; National Natural Science Foundation of China[51861125204] ; Youth Innovation Promotion Association Program - Chinese Academy of Sciences ; Light of the West multidisciplinary team project - Chinese Academy of Sciences |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000641369700005 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/13462]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Li, Zhe |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, CAS Key Lab Reservoir Water Environm, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 3.China Three Gorges Corp, Beijing 100038, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Yan,Cheng, Yuran,He, Pan,et al. New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,774:11. |
| APA | Xiao, Yan,Cheng, Yuran,He, Pan,Wu, Xinghua,&Li, Zhe.(2021).New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves.SCIENCE OF THE TOTAL ENVIRONMENT,774,11. |
| MLA | Xiao, Yan,et al."New insights into external layers of cyanobacteria and microalgae based on multiscale analysis of AFM force-distance curves".SCIENCE OF THE TOTAL ENVIRONMENT 774(2021):11. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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