A trigger-based aggregation of aptamer-functionalized gold nanoparticles for colorimetry: An example on detection of Escherichia coli O157:H7
文献类型：期刊论文
| 作者 | Xie, Yuanyang1,2,3; Huang, Yu1,5 ; Li, Jiye1,5; Wu, Jiangling4
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| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2021-07-15 |
| 卷号 | 339页码:10 |
| 关键词 | Gold nanoparticle Colorimetric sensor Localized surface plasmon resonance pH regulation |
| DOI | 10.1016/j.snb.2021.129865 |
| 通讯作者 | Huang, Yu(huangyu@cigit.ac.cn) ; Wu, Jiangling(wjl135@126.com) |
| 英文摘要 | A pH-triggered colorimetric assay has been established by using aptamer anchored gold nanoparticles to detect Escherichia coli O157:H7 (E. coli O157:H7). Gold nanoparticles (AuNPs) functionalized by aptamer via thiol groups were used as probes (HS-Apt@AuNPs).Solvents of HCl (acid), NaOH (alkaline) and MgCl2 (nearly neutral) were used as triggers to initiate the movement of HS-Apt@AuNPs in the presence of E. coli O157:H7 bacteria respectively. Due to the pH effect of trigger on the surface charges of E. coli O157:H7 and the contribution of aptamer to capture E. coli O157:H7, the movement of HS-Apt@AuNPs with the presence of E. coli O157:H7 exhibit dispersion and aggregation respectively, which leads the change of colorimetric signal to an opposite direction. Taking the solvent of NaCl frequently used as trigger for gold nanoparticle based colorimetric sensor into consideration, the utilization of pH dependent triggers provide an alternative for this kind of trigger-based sensor. Design of gold nanoparticle-based colorimetric biosensing assay suiting a wide range of trigger is more flexible for specific diagnosis. These results demonstrate a great potential to design bacterial sensor with high sensitivity to defend public health. |
| 资助项目 | Natural Science Foundation of Chongqing[cstc2018jcyjAX0718] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0251] |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000647778200001 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/13477]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Huang, Yu; Wu, Jiangling |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Kings Coll London, Dept Phys, London WC2R 2LS, England 3.Kings Coll London, London Ctr Nanotechnol, London WC2R 2LS, England 4.Chongqing Med Univ, Dept Clin Lab, Univ Town Hosp, Chongqing 401331, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yuanyang,Huang, Yu,Li, Jiye,et al. A trigger-based aggregation of aptamer-functionalized gold nanoparticles for colorimetry: An example on detection of Escherichia coli O157:H7[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,339:10. |
| APA | Xie, Yuanyang,Huang, Yu,Li, Jiye,&Wu, Jiangling.(2021).A trigger-based aggregation of aptamer-functionalized gold nanoparticles for colorimetry: An example on detection of Escherichia coli O157:H7.SENSORS AND ACTUATORS B-CHEMICAL,339,10. |
| MLA | Xie, Yuanyang,et al."A trigger-based aggregation of aptamer-functionalized gold nanoparticles for colorimetry: An example on detection of Escherichia coli O157:H7".SENSORS AND ACTUATORS B-CHEMICAL 339(2021):10. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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