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Development of an ELISA for distinguishing convalescent sera with Mycoplasma hyopneumoniae infection from hyperimmune sera responses to bacterin vaccination in pigs

文献类型：期刊论文

作者Ding, Honglei2,3; Wen, Yukang2,3; Xu, Zuobo2,3; Zhou, Bingqian2,3; Tlili, Chaker1; Tian, Yaqin2,3; Wang, Zhaodi2,3; Ning, Yaru2,3; Xin, Jiuqing4
刊名VETERINARY MEDICINE AND SCIENCE
出版日期2021-05-22
页码10
关键词convalescent sera hyperimmune sera IgG indirect ELISA Mycoplasma hyopneumoniae
DOI10.1002/vms3.539
通讯作者Ding, Honglei(hongleiding@swu.edu.cn) ; Xin, Jiuqing(xinjiuqing@caas.cn)
英文摘要Vaccination with inactivated bacterin is the most popular and practical measure to control enzootic pneumonia. After immunisation with inactivated bacterin, Mycoplasma hyopneumoniae colonised on the respiratory tract and lung stimulates the humoural immune responses and produces IgG and IgA antibodies. ELISA is a widely used serological method to detect M. hyopneumoniae antibodies. However, commercial IgG-ELISA kit cannot distinguish between inactivated bacterin-induced hyperimmune sera and convalescent sera stimulated by natural infection. SIgA-ELISA method needs to collect nasal swabs, but collecting nasal swabs is not easy to operate. Establishment of a discriminative ELISA detecting humoural IgG from convalescent sera but not hyperimmune sera facilitates to evaluate the natural infection of M. hyopneumoniae after inactivated bacterin vaccination. We expressed and purified a recombinant protein named Mhp366-N which contains an epitope recognised by the convalescent sera but not hyperimmune sera. The developed discriminative IgG-ELISA could discriminate between inactivated bacterin-induced hyperimmune sera and convalescent sera and was reproducible, sensitive and specific to M. hyopneumoniae antibody produced by natural infection. Compared to SIgA-ELISA method, discriminative IgG-ELISA was more convenient to detect IgG antibody from sera than IgA from nasal swabs, although it has limited sensitivity in the early stages of infection. Additionally, to some extent, it has a potential to avoid the interference of maternally derived IgG antibodies. The established discriminative IgG-ELISA was efficient to judge the serological IgG antibodies induced from natural infection or inactivated vaccine stimulation and provided a useful method to investigate and evaluate the live organism infection after the application of inactivated bacterin.
资助项目State Key Laboratory of Veterinary Biotechnology Foundation[SKLVBF201905] ; Fundamental Research Funds for the Central Universities[XDJK2020B012] ; Chongqing Technology Innovation and Application Development Project[cstc2019jscx--msxmX0402]
WOS研究方向Veterinary Sciences
语种英语
出版者WILEY
WOS记录号WOS:000652797700001
源URL[http://119.78.100.138/handle/2HOD01W0/13565]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Ding, Honglei; Xin, Jiuqing
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China
2.Southwest Univ, Coll Vet Sci, Lab Vet Mycoplasmol, 2 Tiansheng Rd, Chongqing 400715, Peoples R China
3.Southwest Univ, Med Res Inst, Immunol Res Ctr, Chongqing, Peoples R China
4.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Honglei,Wen, Yukang,Xu, Zuobo,et al. Development of an ELISA for distinguishing convalescent sera with Mycoplasma hyopneumoniae infection from hyperimmune sera responses to bacterin vaccination in pigs[J]. VETERINARY MEDICINE AND SCIENCE,2021:10.
APA Ding, Honglei.,Wen, Yukang.,Xu, Zuobo.,Zhou, Bingqian.,Tlili, Chaker.,...&Xin, Jiuqing.(2021).Development of an ELISA for distinguishing convalescent sera with Mycoplasma hyopneumoniae infection from hyperimmune sera responses to bacterin vaccination in pigs.VETERINARY MEDICINE AND SCIENCE,10.
MLA Ding, Honglei,et al."Development of an ELISA for distinguishing convalescent sera with Mycoplasma hyopneumoniae infection from hyperimmune sera responses to bacterin vaccination in pigs".VETERINARY MEDICINE AND SCIENCE (2021):10.

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来源：重庆绿色智能技术研究院

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