Visualizing of AuNPs protection aptamer from DNase I enzyme digestion based on Nanopipette and its use for Microcystin-LR detection
文献类型：期刊论文
| 作者 | Xie, Wanyi2,3 ; He, Shixuan1,2,3; Fang, Shaoxi2,3; Liang, Liyuan2,3; Shi, Biao2,3; Wang, Deqiang2,3
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2021-08-15 |
| 卷号 | 1173页码:7 |
| ISSN号 | 0003-2670 |
| 关键词 | Nanopipette AuNPs DNase I Aptamer Fluorescence Microcystin-LR |
| DOI | 10.1016/j.aca.2021.338698 |
| 通讯作者 | He, Shixuan(heshixuan@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn) |
| 英文摘要 | A simple and effective fluorescence platform has been established for visualizing nanomaterials' protective effect of DNA from cellular enzyme digestion based on nanopipette. In a proof-of-concept trial, gold nanoparticles (AuNPs) protect aptamer was designed, and it used for Microcystin-LR (MC-LR) sensitive detection. In the absence of MC-LR, FAM-labeled aptamers were combined on AuNPs, resulting in weak fluorescence emission. In the presence of MC-LR, aptamer bound with MC-LR. The formed complex leaves the surface of AuNPs. With the addition of the deoxyribonuclease I (DNase I) enzyme, the aptamer was selectively cleavaged, and MC-LR was released as an additional target molecule to achieve signal amplification and obtain strong fluorescence intensity. At the optimized conditions, a wide linear range (0.25 nMe20 nM) of fluorescence response for MC-LR was obtained. Further, by electrochemically manipulation MC-LR and DNase I inside confining nanopipette, which is filled with aptamer/AuNPs. The fluorescence intensity change with the aptamer and AuNPs interaction, these results directly visualize the process of DNA cleavage, and the interaction with AuNPs can effectively prevent the cleavage at the nanoscale confinement. This convenient nanoscale device provides new kinetic information about the dynamic chemical processes at a single-molecule level. (c) 2021 Elsevier B.V. All rights reserved. |
| 资助项目 | Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0308] ; Natural Science Foundation of Chongqing, China[cstc2018jcy-jAX0310] ; National Natural Science Foundation of China[41603089] ; Instrument development program of CAS[YZ201568] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000660288500003 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/13694]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | He, Shixuan; Wang, Deqiang |
| 作者单位 | 1.Sichuan Univ, Phys Dept, Chengdu 610065, Sichuan, Peoples R China 2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Wanyi,He, Shixuan,Fang, Shaoxi,et al. Visualizing of AuNPs protection aptamer from DNase I enzyme digestion based on Nanopipette and its use for Microcystin-LR detection[J]. ANALYTICA CHIMICA ACTA,2021,1173:7. |
| APA | Xie, Wanyi,He, Shixuan,Fang, Shaoxi,Liang, Liyuan,Shi, Biao,&Wang, Deqiang.(2021).Visualizing of AuNPs protection aptamer from DNase I enzyme digestion based on Nanopipette and its use for Microcystin-LR detection.ANALYTICA CHIMICA ACTA,1173,7. |
| MLA | Xie, Wanyi,et al."Visualizing of AuNPs protection aptamer from DNase I enzyme digestion based on Nanopipette and its use for Microcystin-LR detection".ANALYTICA CHIMICA ACTA 1173(2021):7. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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