A Highly Sensitive Label-free Aptasensor Based on Gold Nanourchins and Carbon Nanohorns for the Detection of Lipocalin-2 (LCN-2)
文献类型：期刊论文
| 作者 | Kurup, Chitra Padmakumari1; Mohd-Naim, Noor Faizah3; Tlili, Chaker2 ; Ahmed, Minhaz Uddin1
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| 刊名 | ANALYTICAL SCIENCES
 |
| 出版日期 | 2021-06-01 |
| 卷号 | 37期号:6页码:825-831 |
| 关键词 | LCN-2 aptasensor gold nanourchin carbon nanohorn electrochemical |
| ISSN号 | 0910-6340 |
| DOI | 10.2116/analsci.20P303 |
| 通讯作者 | Ahmed, Minhaz Uddin(minhaz.ahmed@ubd.edu.bn) |
| 英文摘要 | A synergistic nanocomposite film composed of gold nanourchins (AuNU), oxidised carbon nanohorns (CNH), and chitosan functioned as an electrode modifier in the fabrication of the sensitive lipocalin-2 (LCN-2) aptasensor. The AuNUs/CNH/CS composite increased the surface area and thereby amplified the signal transduction. The amine-terminated LCN-2 aptamer was immobilised through the amide bond formed between the carboxyl group of polyglutamic acid (PGA) and the amine group of aptamer. Interaction of LCN-2 with the aptamer caused conformational changes in the structure of the aptamer. This generated higher conductivity, resulting in increased DPV peak current. The DPV signal increased with increasing concentration of LCN-2, and the change in signal was used for quantitative detection. The proposed aptasensor was able to detect LCN-2 in the linear range of 0.1 - 100.0 pg mL(-1), with a low detection limit of 10 fg mL(-1). The aptasensor showed high sensitivity, selectivity, reproducibility, and was able to detect LCN-2 in serum samples. |
| 资助项目 | Ministry of Education of Brunei Darussalam ; Universiti Brunei Darussalam ; Universiti Brunei Darussalam's grant[UBD/RSCH/1.4/FICBF(b)/2018/010] ; Brunei Research Council of Negara Brunei Darussalam[BRC-10] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000661437600006 |
| 出版者 | JAPAN SOC ANALYTICAL CHEMISTRY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/13700]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Ahmed, Minhaz Uddin |
| 作者单位 | 1.Univ Brunei Darussalam, Fac Sci, Biosensors & Nanobiotechnol Lab, Integrated Sci Bldg,Jalan Tungku Link, BE-1410 Gadong, Brunei 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China 3.Univ Brunei Darussalam, PAPRSB Inst Hlth Sci, Jalan Tungku Link, BE-1410 Gadong, Brunei |
| 推荐引用方式 GB/T 7714 | Kurup, Chitra Padmakumari,Mohd-Naim, Noor Faizah,Tlili, Chaker,et al. A Highly Sensitive Label-free Aptasensor Based on Gold Nanourchins and Carbon Nanohorns for the Detection of Lipocalin-2 (LCN-2)[J]. ANALYTICAL SCIENCES,2021,37(6):825-831. |
| APA | Kurup, Chitra Padmakumari,Mohd-Naim, Noor Faizah,Tlili, Chaker,&Ahmed, Minhaz Uddin.(2021).A Highly Sensitive Label-free Aptasensor Based on Gold Nanourchins and Carbon Nanohorns for the Detection of Lipocalin-2 (LCN-2).ANALYTICAL SCIENCES,37(6),825-831. |
| MLA | Kurup, Chitra Padmakumari,et al."A Highly Sensitive Label-free Aptasensor Based on Gold Nanourchins and Carbon Nanohorns for the Detection of Lipocalin-2 (LCN-2)".ANALYTICAL SCIENCES 37.6(2021):825-831. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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