A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle
文献类型:期刊论文
作者 | Yang, Yun2; Wang, Hao2; He, Jia2; Shi, Wenchao2; Jiang, Zhanmei2; Gao, Lina2; Jiang, Yan2; Ni, Rui2; Yang, Qifen2; Luo, Lingfei1,2 |
刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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出版日期 | 2021-06-22 |
卷号 | 118期号:25页码:9 |
关键词 | single-cell labeling and tracing endoderm fate map pancreatic fate cell cycle |
ISSN号 | 0027-8424 |
DOI | 10.1073/pnas.2025793118|1of9 |
通讯作者 | Luo, Lingfei(lluo@swu.edu.cn) |
英文摘要 | A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell-resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at single-cell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination. |
资助项目 | National Natural Science Foundation of China[31730060] ; Initiative Postdocs Supporting Program[CQBX201807] |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
WOS记录号 | WOS:000675808200014 |
出版者 | NATL ACAD SCIENCES |
源URL | [http://119.78.100.138/handle/2HOD01W0/13762] ![]() |
专题 | 中国科学院重庆绿色智能技术研究院 |
通讯作者 | Luo, Lingfei |
作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, AH-400714 Chongqing, Peoples R China 2.Southwest Univ, Inst Dev Biol & Regenerat Med, AH-400715 Chongqing, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Yun,Wang, Hao,He, Jia,et al. A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118(25):9. |
APA | Yang, Yun.,Wang, Hao.,He, Jia.,Shi, Wenchao.,Jiang, Zhanmei.,...&Luo, Lingfei.(2021).A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118(25),9. |
MLA | Yang, Yun,et al."A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118.25(2021):9. |
入库方式: OAI收割
来源:重庆绿色智能技术研究院
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