中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle

文献类型:期刊论文

作者Yang, Yun2; Wang, Hao2; He, Jia2; Shi, Wenchao2; Jiang, Zhanmei2; Gao, Lina2; Jiang, Yan2; Ni, Rui2; Yang, Qifen2; Luo, Lingfei1,2
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2021-06-22
卷号118期号:25页码:9
关键词single-cell labeling and tracing endoderm fate map pancreatic fate cell cycle
ISSN号0027-8424
DOI10.1073/pnas.2025793118|1of9
通讯作者Luo, Lingfei(lluo@swu.edu.cn)
英文摘要A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell-resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at single-cell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination.
资助项目National Natural Science Foundation of China[31730060] ; Initiative Postdocs Supporting Program[CQBX201807]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000675808200014
出版者NATL ACAD SCIENCES
源URL[http://119.78.100.138/handle/2HOD01W0/13762]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Luo, Lingfei
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, AH-400714 Chongqing, Peoples R China
2.Southwest Univ, Inst Dev Biol & Regenerat Med, AH-400715 Chongqing, Peoples R China
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GB/T 7714
Yang, Yun,Wang, Hao,He, Jia,et al. A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118(25):9.
APA Yang, Yun.,Wang, Hao.,He, Jia.,Shi, Wenchao.,Jiang, Zhanmei.,...&Luo, Lingfei.(2021).A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118(25),9.
MLA Yang, Yun,et al."A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118.25(2021):9.

入库方式: OAI收割

来源:重庆绿色智能技术研究院

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