In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au-Pt Nanoparticles
文献类型：期刊论文
| 作者 | Bao, Jing1,2; Yang, Huisi1; Zhao, Jiaying1; Yang, Cheng2; Duan, Yu3; Lu, Wenqiang3 ; Gao, Mingxuan2; Chen, Ming2; Huo, Danqun1; Hou, Changjun1
|
| 刊名 | ACS APPLIED NANO MATERIALS
 |
| 出版日期 | 2021-09-24 |
| 卷号 | 4期号:9页码:9449-9458 |
| 关键词 | in situ detection living cell assay hydrogen peroxide 3D nanoarray Au-Pt nanoparticles |
| ISSN号 | 2574-0970 |
| DOI | 10.1021/acsanm.1c01924 |
| 通讯作者 | Chen, Ming(chming1971@126.com) ; Huo, Danqun(huodq@cqu.edu.cn) ; Hou, Changjun(houcj@cqu.edu.cn) |
| 英文摘要 | In this study, a 3D flexible sensing interface (CC/GWs/Au-Pt) with the direct growth of living cells was constructed for the rapid, sensitive, in situ, and real-time analysis and detection of hydrogen peroxide (H2O2). Similarly, graphene walls (GWs) vertically grew on the flexible and conductive carbon cloth (CC) by RF-PECVD, and Au-Pt nanoparticles were electrodeposited on CC/GWs to build an ultrasensitive sensing interface with good electrocatalytic activity. The proposed CC/GWs/Au-Pt platform shows good electrocatalytic performance for H2O2, and the detection limit was as low as 0.084 pM (84 mu M) with wide linear range (1.5-98 and 148-7148 mu M) and fast response (<5 s). In addition, the cells were able to adhere and grow well on the CC/GWs/Au-Pt sensing electrode, which was attributed to the good biocompatibility of CC/GWs/Au-Pt, providing a 3D microenvironment for the adhesion/growth of cells. Under the stimulation of drugs, this CC/GWs/Au-Pt biosensor achieved the real-time detection of released H(2)O(2 )by cells. The developed cell growth monitoring sensing interface can shorten the diffusion distance between reactive biomolecule and sensing interface, therefore improving the sensitivity and the accuracy of biosensor, which holds great promise for live-cell activity and its metabolites of continuous dynamic monitoring. |
| 资助项目 | National Natural Science Foundation of China[81772290] ; National Natural Science Foundation of China[82002251] ; Graduate Research and Innovation Foundation of Chongqing, China[CYB20070] ; Graduate Research and Innovation Foundation of Chongqing, China[2020CDCGJ014] ; National Facility for Translational Medicine (Shanghai) Open Research Found[TMSK-2021-113] ; Chongqing Science and Technology Commission[CSTC2018jcyjAX0062] ; Chongqing Graduate Tutor Team Construction Project ; Chongqing University ; Analytical and Testing Center of Chongqing University |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000702079900081 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/14140]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Chen, Ming; Huo, Danqun; Hou, Changjun |
| 作者单位 | 1.Chongqing Univ, Bioengn Coll, State & Local Joint Engn Lab Vasc Implants, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 400044, Peoples R China 2.Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Clin Lab Med, Chongqing 400038, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Jing,Yang, Huisi,Zhao, Jiaying,et al. In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au-Pt Nanoparticles[J]. ACS APPLIED NANO MATERIALS,2021,4(9):9449-9458. |
| APA | Bao, Jing.,Yang, Huisi.,Zhao, Jiaying.,Yang, Cheng.,Duan, Yu.,...&Hou, Changjun.(2021).In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au-Pt Nanoparticles.ACS APPLIED NANO MATERIALS,4(9),9449-9458. |
| MLA | Bao, Jing,et al."In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au-Pt Nanoparticles".ACS APPLIED NANO MATERIALS 4.9(2021):9449-9458. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。