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Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor

文献类型：期刊论文

作者Wu, Ji1,2; Wang, Sen1,2; Liang, Liyuan1,2; Zhao, Chuanqi1,3,4; Yin, Yajie1,2; Weng, Ting1,2; Yin, Bohua1,2; Wang, Liang1,2; Wang, Deqiang1,2
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2021-12-01
卷号348页码:9
关键词Solid-state nanopore G-quadruplexes I-motif Duplex Competition
DOI10.1016/j.snb.2021.130712
通讯作者Liang, Liyuan(liangliyuan@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
英文摘要Apart from duplex helix, the coexisted noncanonical DNA may be involved in inhibiting DNA replication, and controlling gene expression. The competition of duplex/ G4/ i-motif depends on many factors, including sequence information, external conditions and binding effects. This work looked into the human telomere sequences of different length (hTelo-1, 2) and intramolecular G-rich and C-rich strands (hTelo-3, 4, 5). Nanopore translocation properties suggested that in the presence of their complementary sequences in the mixed K+/Cs+ buffer, G4 was the major species, and G4 from hTelo-2 that holds multiple G-quartets formed slower than shorter hTelo-1. The intramolecular G/C-rich strands hTelo-3, 4, 5 predominantly formed duplex rather than quadruplex under K+ or acidic conditions, indicating that intramolecular quadruplex is less stable than duplex due to the strand strain. Translocation behaviors of mixed proto-oncogene cMYC/c-cMYC illustrated that G4 was formed in both K+ and Cs+, and Cs+ inhibited the duplex formation in K+/Cs+ buffer. The increase of the ratio of Cs+ will decrease the duplexes species. Our work demonstrated the relative stability of duplex/quadruplex under various conditions with distinct strands, which is useful for the investigation of DNA secondary structural dynamics and transition mechanism in biological function regions, and paved the way for the potential of disease-targeted diagnosis.
资助项目Natural Science Foundation of Beibei, Chongqing ,China[E1316101] ; Excellent Youth Foundation of Chongqing Scientific Committee[cstc2021jcyj-jq0013] ; West Light Foundation of The chinese Academy of Sciences ; National Natural Science Foundation Of China[31800711] ; Natural Science Foundation of Chongqing, China[cstc2020jcyj-msxmX1007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0308] ; Chongqing Talents[CQYC201905007] ; university of the Chinese Academy of Sciences
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000701803600001
出版者ELSEVIER SCIENCE SA
源URL[http://119.78.100.138/handle/2HOD01W0/14158]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Liang, Liyuan; Wang, Deqiang
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China
2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, Lab Chem Biol, Changchun 130022, Jilin, Peoples R China
4.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Jilin, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Ji,Wang, Sen,Liang, Liyuan,et al. Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,348:9.
APA Wu, Ji.,Wang, Sen.,Liang, Liyuan.,Zhao, Chuanqi.,Yin, Yajie.,...&Wang, Deqiang.(2021).Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor.SENSORS AND ACTUATORS B-CHEMICAL,348,9.
MLA Wu, Ji,et al."Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor".SENSORS AND ACTUATORS B-CHEMICAL 348(2021):9.

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来源：重庆绿色智能技术研究院

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