In situ Plasmon-Enhanced CARS and TPEF for Gram staining identification of non-fluorescent bacteria
文献类型：期刊论文
| 作者 | Cui, Lin2; Li, Rui3; Mu, Tingjie1; Wang, Jiangcai4; Zhang, Wei5 ; Sun, Mengtao2
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| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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| 出版日期 | 2022-01-05 |
| 卷号 | 264页码:9 |
| 关键词 | In situ Plasmon-Enhancement CARS TPEF Gram Staining Identification nonfluorescent Bacteria |
| ISSN号 | 1386-1425 |
| DOI | 10.1016/j.saa.2021.120283 |
| 通讯作者 | Zhang, Wei(zhangwei@cigit.ac.cn) ; Sun, Mengtao(mengtaosun@ustb.edu.cn) |
| 英文摘要 | In this work, we report in situ nonlinear microscopic images on plasmon-enhanced coherent anti-Stokes Raman scattering (CARS) and plasmon-Induced two-photon excited fluorescence (TPEF)of non-fluorescent microorganism. Our unique synthesized Au@Ag nanorods provide with two distinct surface-plasmon resonance (SPR) at 400 and 800 nm, respectively, which can efficiently induce linear fluorescence signals of E. coli but also enhance the nonlinear optical spectroscopy signals of TPEF, and coherent anti-Stokes Raman scattering (CARS) imaging of E. coli and S. aureus. Furthermore, calculations with complete active space self-consistent field (CASSCF) reveals the hot electrons of SPs can efficiently induce the biological fluorescence of non-fluorescent flavin nucleotides on the surface of E. coli. This novel mechanism is expected to guide the development and application of new microbial detection reagents. Gram-negative and Gram-positive bacteria can be well distinguished by nonlinear microscopic imaging of the CARS signal at 1589 cm(-1). Benefit by the strong penetrability of non-linear optical signals, it is expected to realize in situ real-time detection and classification of pathogenic microbial infections in vivo. (C) 2021 Elsevier B.V. All rights reserved. |
| 资助项目 | National Science Foundation of China[91436102] ; National Science Foundation of China[11374353] ; Fundamental Research Funds for the Central Universities[06500067] |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000701787800009 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/14168]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Zhang, Wei; Sun, Mengtao |
| 作者单位 | 1.Peoples Hosp Linxia Hui Autonomous Prefecture, Linxia City 731100, Peoples R China 2.Univ Sci & Technol Beijing, Sch Math & Phys, Beijing 100083, Peoples R China 3.Dalian Univ Technol, Sch Phys, Dalian 116023, Peoples R China 4.Tsinghua Univ, State Key Lab Tribol, Beijing 100084, Peoples R China 5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Lin,Li, Rui,Mu, Tingjie,et al. In situ Plasmon-Enhanced CARS and TPEF for Gram staining identification of non-fluorescent bacteria[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2022,264:9. |
| APA | Cui, Lin,Li, Rui,Mu, Tingjie,Wang, Jiangcai,Zhang, Wei,&Sun, Mengtao.(2022).In situ Plasmon-Enhanced CARS and TPEF for Gram staining identification of non-fluorescent bacteria.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,264,9. |
| MLA | Cui, Lin,et al."In situ Plasmon-Enhanced CARS and TPEF for Gram staining identification of non-fluorescent bacteria".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 264(2022):9. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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