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Affinity-Guided Isolation and Identification of Procyanidin B2 from Mangosteen (Garcinia mangostana L.) Rinds and its In Vitro LPS Binding and Neutralization Activities

文献类型：期刊论文

作者Zheng, Xinchuan1; Yang, Yongjun2; Lu, Yongling2; Chen, Qian2
刊名PLANT FOODS FOR HUMAN NUTRITION
出版日期2021-09-04
页码7
关键词Mangosteen Procyanidin B2 LPS Binding Neutralization
ISSN号0921-9668
DOI10.1007/s11130-021-00920-9
通讯作者Zheng, Xinchuan(zhengxinchuan@cigit.ac.cn)
英文摘要Garcinia mangostana L. (mangosteen) is a tropical fruit that has been used for medicinal purposes in Southeast Asia for centuries. With an interest in its applications to treat infection, we sought to investigate the bioactive constituents of mangosteen and identified the phenolic compound procyanidin B2 from the mangosteen pericarp by examining lipopolysaccharide (LPS) binding capacity. The LPS binding and neutralization activities of procyanidin B2 were determined by a combination of biophysical and in silico techniques. The affinity of procyanidin B2 to LPS was 1.61 x 10(-5) M. Procyanidin B2 significantly neutralized LPS and selectively inhibited the LPS-induced release of tumor necrosis factor (TNF)-alpha from RAW264.7 cells in a dose-dependent manner. Binding thermodynamics revealed favorable hydrogen bonding and hydrophobic interactions between procyanidin B2 and LPS. Molecular simulations suggested that hydrogen bonding and hydrophobic interactions were involved in the binding process. These findings have, for the first time, shed light on the anti-inflammatory properties of procyanidin B2 through LPS binding and neutralization and provided a promising lead for the development of antiendotoxin agents.
资助项目National Natural Science Foundation of China[81803394]
WOS研究方向Plant Sciences ; Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种英语
WOS记录号WOS:000693346100001
出版者SPRINGER
源URL[http://119.78.100.138/handle/2HOD01W0/14242]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Zheng, Xinchuan
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
2.Army Med Univ, Affiliated Hosp 1, Clin Res Ctr, Chongqing 400038, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Xinchuan,Yang, Yongjun,Lu, Yongling,et al. Affinity-Guided Isolation and Identification of Procyanidin B2 from Mangosteen (Garcinia mangostana L.) Rinds and its In Vitro LPS Binding and Neutralization Activities[J]. PLANT FOODS FOR HUMAN NUTRITION,2021:7.
APA Zheng, Xinchuan,Yang, Yongjun,Lu, Yongling,&Chen, Qian.(2021).Affinity-Guided Isolation and Identification of Procyanidin B2 from Mangosteen (Garcinia mangostana L.) Rinds and its In Vitro LPS Binding and Neutralization Activities.PLANT FOODS FOR HUMAN NUTRITION,7.
MLA Zheng, Xinchuan,et al."Affinity-Guided Isolation and Identification of Procyanidin B2 from Mangosteen (Garcinia mangostana L.) Rinds and its In Vitro LPS Binding and Neutralization Activities".PLANT FOODS FOR HUMAN NUTRITION (2021):7.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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