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Transcriptome and in silico approaches provide new insights into the mechanism of male reproductive toxicity induced by chronic exposure to DEHP

文献类型：期刊论文

作者Ma, Yan-Bo2,4; Manzoor, Rakia1,2; Jia, Pan-Pan2,4; Bian, Wan-Ping2,4; Hamid, Naima2,4; Xie, Zhuo-Yuan2,4; Pei, De-Sheng3
刊名ENVIRONMENTAL POLLUTION
出版日期2021-11-15
卷号289页码:11
关键词DEHP Progesterone receptor Male zebrafish Reproductive toxicity Chronic exposure
ISSN号0269-7491
DOI10.1016/j.envpol.2021.117944
通讯作者Pei, De-Sheng(peids@cigit.ac.cn)
英文摘要Di-(2-ethylhexyl) phthalate (DEHP) can affect the male reproductive system in vertebrates, but the underlying molecular mechanism is still elusive. Therefore, in this study, we aimed to dig the in-depth mechanism of DEHPinduced reproductive toxicity on male zebrafish via testicular transcriptome using embryo exposed at the environmentally relevant concentration (ERC) of 100 mu g/L for 111 days. Moreover, our results were further confirmed via in silico technique and bioassay experimental in vitro (cell lines) and in vivo (zebrafish). The results showed DEHP exposure could affect male spermatogenesis, altered gonad histology, and reduced egg fertilization rate. Transcriptome analysis identified 1879 significant differentially expressed genes enriched in the exposure group. Twenty-seven genes related to three pathways of reproduction behavior were further validated by qPCR. In silico molecular docking revealed that DEHP and its metabolism bind to the zebrafish progesterone receptor (Pgr), suggesting the potential disruption of DEHP to the normal Pgr signaling. To further validate it, a wild-type Pgr plasmid and its mutants on specific binding sites were constructed. The transfection and microinjection experiment demonstrated that these binding sites mutations of Pgr affected the expression levels of male reproductive toxicity. Taken together, our study provided new insight into the molecular mechanisms of male reproductive toxicity induced by DEHP, and Pgr may serve as an important target binding by DEHP pollution, which needs further study in the future.
资助项目CAS Team Project of the Belt and Road, Chongqing Key Program of Basic Research and Advanced Exploration Project[cstc2019jcyj-zdxmX0035] ; National Natural Science Foundation of China[21407146] ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Program of China-Sri Lanka Joint Center for Water Technology Research and Demonstration by Chinese Academy of Sciences (CAS)/China-Sri Lanka Joint Center for Education and Research by CAS ; International Partnership Program of CAS[121311kysb20190071]
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000696650500007
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.138/handle/2HOD01W0/14290]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Pei, De-Sheng
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China
4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Yan-Bo,Manzoor, Rakia,Jia, Pan-Pan,et al. Transcriptome and in silico approaches provide new insights into the mechanism of male reproductive toxicity induced by chronic exposure to DEHP[J]. ENVIRONMENTAL POLLUTION,2021,289:11.
APA Ma, Yan-Bo.,Manzoor, Rakia.,Jia, Pan-Pan.,Bian, Wan-Ping.,Hamid, Naima.,...&Pei, De-Sheng.(2021).Transcriptome and in silico approaches provide new insights into the mechanism of male reproductive toxicity induced by chronic exposure to DEHP.ENVIRONMENTAL POLLUTION,289,11.
MLA Ma, Yan-Bo,et al."Transcriptome and in silico approaches provide new insights into the mechanism of male reproductive toxicity induced by chronic exposure to DEHP".ENVIRONMENTAL POLLUTION 289(2021):11.

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来源：重庆绿色智能技术研究院

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