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Generation of a novel transgenic marine medaka (Oryzias melastigma) for highly sensitive detection of heavy metals in the environment

文献类型：期刊论文

作者Chen, Ya-Nan2,3; Bian, Wan-Ping3; Liu, Li3; Chen, Xin2; Tang, Min2; Pei, De-Sheng1,3
刊名JOURNAL OF HAZARDOUS MATERIALS
出版日期2021-10-05
卷号419页码:8
ISSN号0304-3894
关键词Oryzias melastigma Metallothionein (mt) Promoter Heavy metals Transgenic marine medaka
DOI10.1016/j.jhazmat.2021.126382
通讯作者Tang, Min(tangmin@hainu.edu.cn) ; Pei, De-Sheng(peids@cigit.ac.cn)
英文摘要As typic priority pollutants in the marine environment, heavy metals can be accumulated in the human body leading to serious environmental and health problems. The metal regulatory elements (MREs) have been identified to be the main functional parts for the response to heavy metals. To develop a convenient biological monitoring tool for the detection of heavy metals in the oceans, we generated a transgenic marine medaka line Tg (OmMT: eGFP) with a truncated metallothionein promoter, which was only 193 bp and drove the expression of eGFP. After Tg(OmMT:eGFP) embryos were treated with four different heavy metals and different concentrations, the results showed that the expression level of eGFP was consistent with that of the endogenous mt. The trans genic embryos are very sensitive to Hg2+, and the fluorescence could be induced in the 0.0002 mu M concentration, which is far lower than the primary water standard. The expression level of eGFP and mt showed a dose-dependent manner to heavy metals concentration. Taken together, the newly established marine medaka is a sensitive, efficient, and convenient tool for monitoring heavy metal pollution in the environment, especially seawater.
资助项目CAS Team Project of the Belt and Road ; Research Program of Chongqing Science and Tech-nology Commission[cstc2019jcyj-zdxmX0035] ; Research Program of Chongqing Science and Tech-nology Commission[cstc2019jcyj-msxm0700] ; Research Program of Chongqing Science and Tech-nology Commission[CSTCCXLJRC201714] ; Research Program of Chongqing Science and Tech-nology Commission[2019-04 Beibei] ; National Nat-ural Science Foundation of China[31660128] ; Hainan Province Basic and Applied Basic Research Program (Natural Science) High Level Talent Project[2019RC039] ; Scientific Research Projects of Colleges and Universities in Hainan Province[Hnky2019-2] ; Program of China-Sri Lanka Joint Center for Water Technology Research and Demonstration by Chinese Academy of Sciences (CAS) /China-Sri Lanka Joint Center for Education and Research by CAS ; International Partnership Program of CAS[121311kysb20190071]
WOS研究方向Engineering ; Environmental Sciences & Ecology
语种英语
出版者ELSEVIER
WOS记录号WOS:000696614600001
源URL[http://119.78.100.138/handle/2HOD01W0/14294]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Tang, Min; Pei, De-Sheng
作者单位1.Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China
2.Hainan Univ, Coll Ecol & Environm, Key Lab Minist Educ Adv Mat Trop Isl Resources, Haikou 570228, Hainan, Peoples R China
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Ya-Nan,Bian, Wan-Ping,Liu, Li,et al. Generation of a novel transgenic marine medaka (Oryzias melastigma) for highly sensitive detection of heavy metals in the environment[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,419:8.
APA Chen, Ya-Nan,Bian, Wan-Ping,Liu, Li,Chen, Xin,Tang, Min,&Pei, De-Sheng.(2021).Generation of a novel transgenic marine medaka (Oryzias melastigma) for highly sensitive detection of heavy metals in the environment.JOURNAL OF HAZARDOUS MATERIALS,419,8.
MLA Chen, Ya-Nan,et al."Generation of a novel transgenic marine medaka (Oryzias melastigma) for highly sensitive detection of heavy metals in the environment".JOURNAL OF HAZARDOUS MATERIALS 419(2021):8.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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