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Nanopore detects.-radiation inhibited HIV-1 protease activity

文献类型：期刊论文

作者Li, Minghan1,3,4; Li, Wei1,4; Xiao, Yicen1,4; Liu, Qianshan1,4; Liang, Liyuan1,4; Wang, Deqiang1,4; Huang, Wenli5; Wang, Liang1,2,4
刊名BIOSENSORS & BIOELECTRONICS
出版日期2021-12-15
卷号194页码:5
ISSN号0956-5663
关键词HIV-1 protease GagPol precursor Enzyme inactivation Co-60 gamma-radiation Nanopore
DOI10.1016/j.bios.2021.113602
通讯作者Huang, Wenli(wenlih11@126.com) ; Wang, Liang(wangliang83@cigit.ac.cn)
英文摘要Inhibition of HIV-1 protease (PR) activity is realized by exposure to Co-60 gamma-radiation. The radiation effects on enzyme kinetics of HIV-1 PR are subsequently monitored using nanopore sensor. Substantial loss of proteolytic efficiency towards GagPol polypeptide is observed due to the radiation treatment. Results shows similar to 50% of GagPol polypeptide was not involved in HIV-1 PR proteolysis by exposure to ultra-low intensity of gamma-radiation (0.1K Gy), and the values reach to over 90% with high gamma-ray treatment. Besides, the inactivation effect is also verified in blood samples which contain the virus protease. Our finding provides the potential benefits of gamma-radiation to inactivate viral proteinic function, and might be a complementary to the designation of HIV-1 PR inhibitors.
资助项目National Natural Science Foundation of China[31800711] ; Chongqing Talents[CQYC201905007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0304] ; Foundation for Subject Construction of Sichuan Academy of Agricultural Sciences[2021XKJS058] ; Pioneer Hundred Talents Program of Chinese Academy of Sciences ; University of Chinese Academy of Sciences
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
出版者ELSEVIER ADVANCED TECHNOLOGY
WOS记录号WOS:000697620500002
源URL[http://119.78.100.138/handle/2HOD01W0/14317]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Huang, Wenli; Wang, Liang
作者单位1.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
2.Chongqing Key Lab Intelligent Med Engn Hepatopanc, Chongqing 401147, Peoples R China
3.Chongqing Univ Posts & Telecommun, Sch Optoelect Engn, Chongqing 400065, Peoples R China
4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
5.Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu 610066, Peoples R China
推荐引用方式
GB/T 7714		 Li, Minghan,Li, Wei,Xiao, Yicen,et al. Nanopore detects.-radiation inhibited HIV-1 protease activity[J]. BIOSENSORS & BIOELECTRONICS,2021,194:5.
APA Li, Minghan.,Li, Wei.,Xiao, Yicen.,Liu, Qianshan.,Liang, Liyuan.,...&Wang, Liang.(2021).Nanopore detects.-radiation inhibited HIV-1 protease activity.BIOSENSORS & BIOELECTRONICS,194,5.
MLA Li, Minghan,et al."Nanopore detects.-radiation inhibited HIV-1 protease activity".BIOSENSORS & BIOELECTRONICS 194(2021):5.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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