Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores
文献类型：期刊论文
| 作者 | Wang, Han1,3,4; Huang, Wenli2; Wang, Yunjiao3,4 ; Li, Wei3,4; Liu, Qianshan3,4; Yin, Bohua3; Liang, Liyuan3,4; Wang, Deqiang3,4; Guan, Xiyun2; Wang, Liang1,3,4
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| 刊名 | ACS SENSORS
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| 出版日期 | 2021-10-22 |
| 卷号 | 6期号:10页码:3781-3788 |
| 关键词 | transmembrane pore HIV-1 Tat protein enzymatic proteolysis copper calcium molecular transport |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.1c01717 |
| 通讯作者 | Guan, Xiyun(xguan5@iit.edu) ; Wang, Liang(wangliang83@cigit.ac.cn) |
| 英文摘要 | HIV-1 Tat protein, an intercellular transporter with a determinant function of delivering "information-rich" molecules in viral multiplication, was tryptic-hydrolyzed and real-time single molecule-monitored in a transmembrane pore. The electrokinetic studies revealed the catalytic and inhibitory effects on enzymatic digestion associated with Ca2+ and Cu2+ ions, respectively, in response to binding interactions with trypsin. Our strategy permits accurate and distinguishable sensing of Ca2+ and Cu2+ via an enzyme assay. In addition, considering the closer mimic of the real situation of HIV spread, measurements in the serum and on cells were also investigated. Transmembrane current measurements together with fluorescence microscopy imaging indicated the potential to perturb the Tat transport in the serum environment and on cells. Because the involved Tat proteolysis should prevent the occurrence of viral delivery, the presented method probably enables efficient hindrance to HIV-1 infection, in complementary to current traditional treatments. |
| 资助项目 | National Natural Science Foundation of China[31800711] ; Chongqing Talents[CQYC201905007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0304] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000711034500030 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/14428]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Guan, Xiyun; Wang, Liang |
| 作者单位 | 1.Chongqing Key Lab Intelligent Med Engn Hepatopanc, Chongqing 401147, Peoples R China 2.IIT, Dept Chem, Chicago, IL 60616 USA 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Han,Huang, Wenli,Wang, Yunjiao,et al. Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores[J]. ACS SENSORS,2021,6(10):3781-3788. |
| APA | Wang, Han.,Huang, Wenli.,Wang, Yunjiao.,Li, Wei.,Liu, Qianshan.,...&Wang, Liang.(2021).Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores.ACS SENSORS,6(10),3781-3788. |
| MLA | Wang, Han,et al."Enzyme Hinders HIV-1 Tat Viral Transport and Real-Time Measured with Nanopores".ACS SENSORS 6.10(2021):3781-3788. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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