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An electrochemical aptasensor for highly sensitive detection of CEA based on exonuclease III and hybrid chain reaction dual signal amplification

文献类型：期刊论文

作者Niu, Cui2; Lin, Xiaojuan2; Jiang, Xin1; Guo, Fei5; Liu, Jianxiao2; Liu, Xiangqin2; Huang, Hongge2; Huang, Yu3,4
刊名BIOELECTROCHEMISTRY
出版日期2022-02-01
卷号143页码:7
关键词Carcinoembryonic antigen Electrochemical aptasensor Exonuclease III Hybrid chain reaction Signal amplifification
ISSN号1567-5394
DOI10.1016/j.bioelechem.2021.107986
通讯作者Huang, Yu(huangyu@cigit.ac.cn)
英文摘要At present, carcinoembryonic antigen (CEA) is considered a broad-spectrum cancer biomarker, and its accurate analysis in clinical samples can assist early cancer diagnosis and treatment. Herein, a novel electrochemical aptasensor has been proposed for CEA detection based on exonuclease III and hybrid chain reaction. The target CEA specifically binds to the aptamer region in hairpin probe 1 (defined as H1) by strong attraction, which leads the rest of the H1 triggering catalytic hairpin assembly to form a high quantity of H1 and hairpin probe 2 (defined as H2) double chain complex (denoted as H1@H2). Subsequently, the exonuclease III digests the complex of H1@H2 and liberates H1 to induce the first signal amplification. Simultaneously, a large number of generated trigger chains initiate a hybrid chain reaction and produce a second signal amplification. This proposed sensor exhibited excellent analytical performance for the detection of CEA, with wide linear range from 10 pg.mL(-1) to 100 ng.mL(-1) and low limit of detection of 0.84 pg.mL(-1). Additionally, the biosensing strategy was successfully verified for direct measurement of CEA in human serum. Therefore, this elaborated sensor provides a new simple method for detecting CEA and exhibits great promise in the early screening of cancer. (C) 2021 Elsevier B.V. All rights reserved.
资助项目The Key Research and Development Program Project Of Xingtai City and The Health and Medical Field of The Third Hospital of Xingtai[ZC20301] ; The Key Research and Development Program Project Of Xingtai City and The Health and Medical Field of The Third Hospital of Xingtai[2020ZC359]
WOS研究方向Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry
语种英语
WOS记录号WOS:000719358200005
出版者ELSEVIER SCIENCE SA
源URL[http://119.78.100.138/handle/2HOD01W0/14547]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Huang, Yu
作者单位1.Vet Gen Hosp Hebei, Pharm Dept, Xingtai 054100, Hebei, Peoples R China
2.Third Hosp Xingtai, Dept Clin Lab, Xingtai 054100, Hebei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
5.Med Coll Xingtai, Dept Basic Med, Xingtai 054100, Hebei, Peoples R China
推荐引用方式
GB/T 7714		 Niu, Cui,Lin, Xiaojuan,Jiang, Xin,et al. An electrochemical aptasensor for highly sensitive detection of CEA based on exonuclease III and hybrid chain reaction dual signal amplification[J]. BIOELECTROCHEMISTRY,2022,143:7.
APA Niu, Cui.,Lin, Xiaojuan.,Jiang, Xin.,Guo, Fei.,Liu, Jianxiao.,...&Huang, Yu.(2022).An electrochemical aptasensor for highly sensitive detection of CEA based on exonuclease III and hybrid chain reaction dual signal amplification.BIOELECTROCHEMISTRY,143,7.
MLA Niu, Cui,et al."An electrochemical aptasensor for highly sensitive detection of CEA based on exonuclease III and hybrid chain reaction dual signal amplification".BIOELECTROCHEMISTRY 143(2022):7.

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来源：重庆绿色智能技术研究院

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