Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment
文献类型：期刊论文
| 作者 | Zhang, Rui2,3; You, Qiannan2,3; Cheng, Mingming2,3; Ge, Mingfeng3; Mei, Qian3; Yang, Li4,5; Dong, Wen-Fei3; Chang, Zhimin1,3 |
| 刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
 |
| 出版日期 | 2021-11-11 |
| 卷号 | 9页码:11 |
| 关键词 | CTCs early diagnosis and treatment electrochemical cytosensor non-invasive release multifunctional Au nanoparticles |
| ISSN号 | 2296-4185 |
| DOI | 10.3389/fbioe.2021.783661 |
| 通讯作者 | Yang, Li(ylyhp@126.com) ; Chang, Zhimin(changzm@sibet.ac.cn) |
| 英文摘要 | Circulating tumor cells (CTCs) are metastatic tumor cells that shed into the blood from solid primary tumors, and their existence significantly increases the risk of metastasis and recurrence. The timely discovery and detection of CTCs are of considerable importance for the early diagnosis and treatment of metastasis. However, the low number of CTCs hinders their detection. In the present study, an ultrasensitive electrochemical cytosensor for specific capture, quantitative detection, and noninvasive release of EpCAM-positive tumor cells was developed. The biosensor was manufactured using gold nanoparticles (AuNPs) to modify the electrode. Three types of AuNPs with controllable sizes and conjugated with a targeting molecule of monoclonal anti-EpCAM antibody were used in this study. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) of the cytosensors were performed to evaluate the cell capture efficiency and performance. The captured 4T1 cells by the AuNPs hindered electron transport efficiency, resulting in increased EIS responses. The cell capture response recorded using EIS or DPV indicated that the optimal AuNPs size should be 17 nm. The cell capture response changed linearly with the concentration range from 8.0 x 10 to 1 x 10(7) cells/mL, and the limit of detection was 50 cells/mL. After these measurements, glycine-HCl (Gly-HCl) was used as an antibody eluent to destroy the binding between antigen and antibody to release the captured tumor cells without compromising their viability for further clinical research. This protocol realizes rapid detection of CTCs with good stability, acceptable assay precision, significant fabrication reproducibility with a relative standard deviation of 2.09%, and good recovery of cells. Our results indicate that the proposed biosensor is promising for the early monitoring of CTCs and may help customize personalized treatment options. |
| 资助项目 | National Key R&D Program of China[2020YFC2004500] ; National Natural Science Foundation of China[81771982] ; National Natural Science Foundation of China[91959112] ; National Natural Science Foundation of China[21803075] ; National Natural Science Foundation of China[81902166] ; Science Foundation of the Chinese Academy of Sciences[2020SYHZ0041] ; Instrument Developing Project of Chinese Academy of Science[YJKYYQ20200038] ; Science and Technology Department of Jinan City[2018GXRC016] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000726328300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/14589]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Yang, Li; Chang, Zhimin |
| 作者单位 | 1.Jinan Guokeyigong Sci & Technol Dev Co Ltd, Jinan, Peoples R China 2.Univ Sci & Technol China, Sch Biomed Engn Suzhou, Div Life Sci & Med, Hefei, Peoples R China 3.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou, Peoples R China 4.Mianyang Teachers Coll, Coll Life Sci & Biotechinol, Mianyang, Sichuan, Peoples R China 5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Rui,You, Qiannan,Cheng, Mingming,et al. Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021,9:11. |
| APA | Zhang, Rui.,You, Qiannan.,Cheng, Mingming.,Ge, Mingfeng.,Mei, Qian.,...&Chang, Zhimin.(2021).Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,9,11. |
| MLA | Zhang, Rui,et al."Multifunctional Gold Nano-Cytosensor With Quick Capture, Electrochemical Detection, and Non-Invasive Release of Circulating Tumor Cells for Early Cancer Treatment".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 9(2021):11. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。