Radiation induces acid tolerance of Clostridium tyrobutyricum and enhances bioproduction of butyric acid through a metabolic switch
文献类型：期刊论文
| 作者 | Zhou,Xiang1 ; Lu,Xi-Hong1; Li,Xue-Hu1; Xin,Zhi-Jun1; Xie,Jia-Rong2; Zhao,Mei-Rong1; Wang,Liang1; Du,Wen-Yue1; Liang,Jian-Ping1
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| 刊名 | Biotechnology for Biofuels
 |
| 出版日期 | 2014-02-18 |
| 卷号 | 7期号:1页码:22-22 |
| 关键词 | Acid inhibition Butyrate Clostridium tyrobutyricum 12C6+ heavy ion Fermentation Irradiation |
| ISSN号 | 1754-6834 |
| DOI | 10.1186/1754-6834-7-22 |
| 其他题名 | Radiation induces acid tolerance of Clostridium tyrobutyricum and enhances bioproduction of butyric acid through a metabolic switch |
| 英文摘要 | AbstractBackgroundButyric acid as a renewable resource has become an increasingly attractive alternative to petroleum-based fuels. Clostridium tyrobutyricum ATCC 25755T is well documented as a fermentation strain for the production of acids. However, it has been reported that butyrate inhibits its growth, and the accumulation of acetate also inhibits biomass synthesis, making production of butyric acid from conventional fermentation processes economically challenging. The present study aimed to identify whether irradiation of C. tyrobutyricum cells makes them more tolerant to butyric acid inhibition and increases the production of butyrate compared with wild type.ResultsIn this work, the fermentation kinetics of C. tyrobutyricum cultures after being classically adapted for growth at 3.6, 7.2 and 10.8?g·L-1 equivalents were studied. The results showed that, regardless of the irradiation used, there was a gradual inhibition of cell growth at butyric acid concentrations above 10.8?g·L-1, with no growth observed at butyric acid concentrations above 3.6?g·L-1 for the wild-type strain during the first 54?h of fermentation. The sodium dodecyl sulfate polyacrylamide gel electrophoresis also showed significantly different expression levels of proteins with molecular mass around the wild-type and irradiated strains. The results showed that the proportion of proteins with molecular weights of 85 and 106?kDa was much higher for the irradiated strains. The specific growth rate decreased by 50% (from 0.42 to 0.21?h-1) and the final concentration of butyrate increased by 68% (from 22.7 to 33.4?g·L-1) for the strain irradiated at 114 AMeV and 40?Gy compared with the wild-type strains.ConclusionsThis study demonstrates that butyric acid production from glucose can be significantly improved and enhanced by using 12C6+ heavy ion-irradiated C. tyrobutyricum. The approach is economical, making it competitive compared with similar fermentation processes. It may prove useful as a first step in a combined method employing long-term continuous fermentation of acid-production processes. |
| WOS关键词 | FIBROUS-BED BIOREACTOR ; HEAVY-ION IRRADIATION ; HYDROGEN-PRODUCTION ; BUTANOL PRODUCTION ; CELL-SURVIVAL ; SACCHAROMYCES-CEREVISIAE ; EXTRACTIVE FERMENTATION ; ALCOHOL-DEHYDROGENASE ; GENUS CLOSTRIDIUM ; ENERGY EFFICIENCY |
| 资助项目 | Hundred Talent Program of the Chinese Academy of Science[O861010ZYO] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1754-6834-7-22 |
| 出版者 | BioMed Central |
| 源URL | [http://119.78.100.186/handle/113462/24463]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Zhou,Xiang; Liang,Jian-Ping |
| 作者单位 | 1.Chinese Academy of Sciences; Institute of Modern Physics 2.China Pharmaceutical University |
| 推荐引用方式 GB/T 7714 | Zhou,Xiang,Lu,Xi-Hong,Li,Xue-Hu,et al. Radiation induces acid tolerance of Clostridium tyrobutyricum and enhances bioproduction of butyric acid through a metabolic switch[J]. Biotechnology for Biofuels,2014,7(1):22-22. |
| APA | Zhou,Xiang.,Lu,Xi-Hong.,Li,Xue-Hu.,Xin,Zhi-Jun.,Xie,Jia-Rong.,...&Liang,Jian-Ping.(2014).Radiation induces acid tolerance of Clostridium tyrobutyricum and enhances bioproduction of butyric acid through a metabolic switch.Biotechnology for Biofuels,7(1),22-22. |
| MLA | Zhou,Xiang,et al."Radiation induces acid tolerance of Clostridium tyrobutyricum and enhances bioproduction of butyric acid through a metabolic switch".Biotechnology for Biofuels 7.1(2014):22-22. |
入库方式： OAI收割
来源：近代物理研究所
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