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CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer

文献类型：期刊论文

作者Li, Hongbin1,2,3,4; Jin, Xiaodong1,2,3,4; Liu, Bingtao1,2,3,4; Zhang, Pengcheng1,2,3,4; Chen, Weiqiang1,2,3,4; Li, Qiang1,2,3,4
刊名BMC CANCER
出版日期2019-08-22
卷号19期号:1页码:14
关键词Colon cancer cells Circular RNA CBL 11 MiR-6778-5p YWHAE
ISSN号1471-2407
DOI10.1186/s12885-019-6017-2
其他题名CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer
通讯作者Li, Qiang(liqiang@impcas.ac.cn)
英文摘要Background Radiotherapy (RT) is considered an important therapeutic strategy in the fight against colorectal cancer (CRC). However, the existence of some radioresistance factors becomes the main challenge for the RT. Recently, non-coding RNAs (ncRNAs) have shown an important role in modulating cancer cell responses to ionizing radiation (IR). It is therefore of great significance to elucidate the exact mechanisms of ncRNAs in IR-mediated responses to CRC. Methods Microarrays were used to identify specific miRNAs that may be altered in response to IR. Bioinformatics, luciferase reporter analyses were used to explore the targets of miR-6778-5p. CircRNA CBL.11 was identified to bind with miR-6778-5p by bioinformatic analysis, AGO2 immunoprecipitation and biotinylated RNA pull-down assay. Functional experiments, including CCK-8 assay, cell colony formation assay and EdU incorporation were conducted to investigate the biological roles of miR-6778-5p and circular RNA CBL.11. Results MiR-6778-5p was suppressed in CRC cells after irradiation. Results of functional experiments indicated that miR-6778-5p promoted the proliferation of CRC cells. Luciferase reporter analyses showed that YWHAE was a target of miR-6778-5p, which mediated the function of miR-6778-5p in the proliferation of CRC cells via the p53 pathway. Furthermore, we have noticed that after carbon ion irradiation, circRNA CBL.11 was increased in CRC cells and could function as a competing endogenous RNA (ceRNA) to regulate YWHAE expression by sponging miR-6778-5p, resulting in regulation the proliferation of CRC cells. Conclusion CircRNA CBL.11 may play an important role in improving the efficacy of carbon ion RT against CRC.
WOS关键词INDUCED DNA-DAMAGE ; UP-REGULATION ; NONCODING RNA ; RADIATION ; METASTASIS ; EXPRESSION ; CARCINOMA ; P53 ; RADIOSENSITIVITY ; RADIORESISTANCE
资助项目National Key Research and Development Program of China[2018YFC0115700] ; National Key Research and Development Program of China[2018YFC0115702] ; National Natural Science Foundation of China[11875299] ; National Natural Science Foundation of China[U1532264]
WOS研究方向Oncology
语种英语
WOS记录号WOS:000483314400005
出版者BMC
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/132772]  
专题中国科学院近代物理研究所
通讯作者Li, Qiang
作者单位1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China
4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Li, Hongbin,Jin, Xiaodong,Liu, Bingtao,et al. CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer[J]. BMC CANCER,2019,19(1):14.
APA Li, Hongbin,Jin, Xiaodong,Liu, Bingtao,Zhang, Pengcheng,Chen, Weiqiang,&Li, Qiang.(2019).CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.BMC CANCER,19(1),14.
MLA Li, Hongbin,et al."CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer".BMC CANCER 19.1(2019):14.

入库方式： OAI收割

来源：近代物理研究所

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