Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*
文献类型：期刊论文
| 作者 | Jia Rong1,2,4; Chen Ya Xiong1,4; Du Ya Rong1,4; Hu Bu Rong1,2,3,4 |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2021-07-01 |
| 卷号 | 34期号:7页码:540-551 |
| 关键词 | MSD assay Low or high LET irradiation Mouse plasma cytokines Dose estimation model |
| ISSN号 | 0895-3988 |
| DOI | 10.3967/bes2021.074 |
| 通讯作者 | Hu Bu Rong(brhu@wmu.edu.cn) |
| 英文摘要 | Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer (LET) irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation. Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery (MSD), a high-throughput and sensitive electrochemiluminescence measurement technique. Dose estimation equations were set up using multiple linear regression analysis. Results The relative levels of IL-6 at 1 h, IL-5 and IL-6 at 24 h, and IL-5, IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently. The minimum measured levels of IL-5, IL-6 and IL-15 were up to 4.0076 pg/mL, 16.4538 pg/mL and 0.4150 pg/mL, respectively. In addition, dose estimation models were established and verified. Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5, IL-6 and IL-15. These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators. Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism. |
| WOS关键词 | CHROMOSOME-ABERRATION ANALYSIS ; MICRONUCLEUS ASSAY ; RADIATION ; BIODOSIMETRY ; EXPOSURE ; ELISA ; MODULATION ; WORKERS ; HUMANS ; CANCER |
| 资助项目 | National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11705248] ; National Natural Science Foundation of China[U1832101] ; National Key Research and Development Program of China[2017YFC0108605] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; Science and Technology Research Project of Gansu Province[17JR5RA307] ; Heavy Ion Research Facility in Lanzhou[Y9HIRFL200] |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:000691530100004 |
| 出版者 | CHINESE CENTER DISEASE CONTROL & PREVENTION |
| 资助机构 | National Natural Science Foundation of China ; National Key Research and Development Program of China ; Science and Technology Research Project of Gansu Province ; Heavy Ion Research Facility in Lanzhou |
| 源URL | [http://119.78.100.186/handle/113462/136381]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Hu Bu Rong |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100039, Peoples R China 3.Wenzhou Med Univ, Sch Publ Hlth & Management, Dept Radiat Med, Wenzhou 325035, Zhejiang, Peoples R China 4.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia Rong,Chen Ya Xiong,Du Ya Rong,et al. Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2021,34(7):540-551. |
| APA | Jia Rong,Chen Ya Xiong,Du Ya Rong,&Hu Bu Rong.(2021).Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,34(7),540-551. |
| MLA | Jia Rong,et al."Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 34.7(2021):540-551. |
入库方式： OAI收割
来源：近代物理研究所
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