Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer
文献类型：期刊论文
| 作者 | Tian Yun-ling4; Fu Song-bo4; Li Bo4; Yuan Ling-yan4; Bing Zhi-tong1,2,3
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| 刊名 | CHINESE JOURNAL OF INTEGRATIVE MEDICINE
 |
| 出版日期 | 2021-09-21 |
| 页码 | 9 |
| 关键词 | network pharmacology prognosis tumor microenvironment Fuzheng Kang'ai Formula Chinese medicine lung adenocarcinoma |
| ISSN号 | 1672-0415 |
| DOI | 10.1007/s11655-021-3451-1 |
| 通讯作者 | Tian Yun-ling(yunlingtian7318@126.com) |
| 英文摘要 | Objective: To study the mechanism of Chinese herbal medicine Fuzheng Kang'ai Formula (FZKA) on tumor microenvironment (TME). Methods: CIBERSORTx was used for analysis of TME. Traditional Chinese Medicine Systems Pharmacology and Analysis Platform was applied to identify compounds-targets network and the Cancer Genome Atlas (TCGA) was employed to identify the differential expression genes (DEGs) between tumor and paracancerous tissues in lung adenocarcinoma (LUAD) from TCGA-LUAD. Additionally, DEGs with prognosis in LUAD was calculated by univariable and multivariate Cox regression. The core targets of FZKA were analyzed in lung adenocarcinoma TME. Protein-protein interaction database was employed to predict down-stream of target. Quantitative reverse transcription polymerase chain reaction was employed for biological experiment in A549, H1299 and PC9 cell lines. Results: The active and resting mast cells were significantly associated with prognosis of LUAD (P<0.05). Of the targets, CCNA2 as an important target of FZKA (hazard ratio=1.41, 95% confidential interval: 1.01-2.01, P<0.05) was a prognostic target and significantly associated with mast cells. CCNA2 was positively correlated with mast cell activation and negatively correlated with mast cell resting state. BCL1L2, ACTL6A and ITGAV were down-stream of CCNA2, which were validated by qRT-PCR in A549 cell. Conclusion: FZKA could directly bind to CCNA2 and inhibit tumor growth by regulating CCNA2 downstream genes and TME of NSCLC closely related to CCNA2. |
| WOS关键词 | CHINESE HERBAL MEDICINE ; ALTERNATIVE MEDICINE ; COMPLEMENTARY ; NETWORK ; BLOCKADE ; THERAPY ; QUALITY |
| WOS研究方向 | Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000698067400004 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.186/handle/113462/136533]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Tian Yun-ling |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Computat Phys, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, Evidence Based Med Ctr, Sch Basic Med Sci, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Dept Endocrinol & Metab, Hosp 1, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian Yun-ling,Fu Song-bo,Li Bo,et al. Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2021:9. |
| APA | Tian Yun-ling,Fu Song-bo,Li Bo,Yuan Ling-yan,&Bing Zhi-tong.(2021).Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,9. |
| MLA | Tian Yun-ling,et al."Mechanism of Fuzheng Kang'ai Formula Regulating Tumor Microenvironment in Non-Small Cell Lung Cancer".CHINESE JOURNAL OF INTEGRATIVE MEDICINE (2021):9. |
入库方式： OAI收割
来源：近代物理研究所
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