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Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process

文献类型：期刊论文

作者Dong, Miaoyin1; Wang, Shuyang1; Xu, Fuqiang1; Wang, Junkai3; Yang, Ning3; Li, Qiaoqiao1; Chen, Jihong1; Li, Wenjian1
刊名BIOTECHNOLOGY FOR BIOFUELS
出版日期2019
卷号12期号:1页码:11
关键词Sweet sorghum straw Pretreatment Structural characterization Enzymatic hydrolysis process Visualization
ISSN号1754-6834
DOI10.1186/s13068-019-1613-6
其他题名Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process
通讯作者Wang, Shuyang(wangsy@impcas.ac.cn) ; Yang, Ning()
英文摘要Background The efficient utilization of lignocellulosic biomass for biofuel production has received increasing attention. Previous studies have investigated the pretreatment process of biomass, but the detailed enzymatic hydrolysis process of pretreated biomass remains largely unclear. Thus, this study investigated the pretreatment efficiency of dilute alkali, acid, hydrogen peroxide and its ultimate effects on enzymatic hydrolysis. Furthermore, to better understand the enzymatic digestion process of alkali-pretreated sweet sorghum straw (SSS), multimodal microscopy techniques were used to visualize the enzymatic hydrolysis process. Result After pretreatment with alkali, an enzymatic hydrolysis efficiency of 86.44% was obtained, which increased by 99.54% compared to the untreated straw (43.23%). The FTIR, XRD and SEM characterization revealed a sequence of microstructural changes occurring in plant cell walls after pretreatment, including the destruction of lignin-polysaccharide interactions, the increase of porosity and crystallinity, and reduction of recalcitrance. During the course of hydrolysis, the cellulase dissolved the cell walls in the same manner and the digestion firstly occurred from the middle of cell walls and then toward the cell wall corners. The CLSM coupled with fluorescent labeling demonstrated that the sclerenchyma cells and vascular bundles in natural SSS were highly lignified, which caused the nonproductive bindings of cellulase on lignin. However, the efficient delignification significantly increased the accessibility and digestibility of cellulase to biomass, thereby improving the saccharification efficiency. Conclusion This work will be helpful in investigating the biomass pretreatment and its structural characterization. In addition, the visualization results of the enzymatic hydrolysis process of pretreated lignocellulose could be used for guidance to explore the lignocellulosic biomass processing and large-scale biofuel production.
WOS关键词LIGNOCELLULOSIC BIOMASS ; ALKALINE PRETREATMENT ; HYDROGEN-PEROXIDE ; DILUTE-ACID ; RICE STRAW ; CELLULOSE ; LIGNIN ; WATER ; SACCHARIFICATION ; BIOCONVERSION
资助项目[National Natural Science Foundation of China] ; [key projects of the Chinese Academy of Sciences]
WOS研究方向Biotechnology & Applied Microbiology ; Energy & Fuels
语种英语
WOS记录号WOS:000499240700002
出版者BMC
资助机构National Natural Science Foundation of China ; key projects of the Chinese Academy of Sciences
源URL[http://119.78.100.186/handle/113462/136886]  
专题中国科学院近代物理研究所
作者单位1.Institute of Modern Physics, Chinese Academy of Sciences
2.Gansu Acad Sci
3.Northwest Normal University - China
4.University of Chinese Academy of Sciences, CAS
推荐引用方式
GB/T 7714		 Dong, Miaoyin,Wang, Shuyang,Xu, Fuqiang,et al. Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12(1):11.
APA Dong, Miaoyin.,Wang, Shuyang.,Xu, Fuqiang.,Wang, Junkai.,Yang, Ning.,...&Li, Wenjian.(2019).Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process.BIOTECHNOLOGY FOR BIOFUELS,12(1),11.
MLA Dong, Miaoyin,et al."Pretreatment of sweet sorghum straw and its enzymatic digestion: insight into the structural changes and visualization of hydrolysis process".BIOTECHNOLOGY FOR BIOFUELS 12.1(2019):11.

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来源：近代物理研究所

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