Identification of robust diagnostic and prognostic gene signatures in different grades of gliomas: a retrospective study
文献类型：期刊论文
| 作者 | Liu, Jieting1,2,9; Zhang, Hongrui3; Zhang, Jingyun4,5; Bing, Zhitong6,7 ; Wang, Yingbin1; Li, Qiao8; Yang, Kehu2,9
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| 刊名 | PEERJ
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| 出版日期 | 2021-05-11 |
| 卷号 | 9页码:23 |
| 关键词 | Gliomas Bioinformatics Gene Signatures Prognosis LASSO |
| ISSN号 | 2167-8359 |
| DOI | 10.7717/peerj.11350 |
| 通讯作者 | Yang, Kehu(ke-huyangebm2006@126.com) |
| 英文摘要 | Background. Gliomas are the most common primary tumors of the central nervous system. The complexity and heterogeneity of the tumor makes it difficult to obtain good biomarkers for drug development. In this study, through The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA), we analyze the common diagnostic and prognostic moleculer markers in Caucasian and Asian populations, which can be used as drug targets in the future. Methods. The RNA-seq data from Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) were analyzed to identify signatures. Based on the signatures, the prognosis index (PI) of every patient was constructed to predict the prognostic risk. Also, gene ontology (GO) functional enrichment analysis and KEGG analysis were conducted to investigate the biological functions of these mRNAs. Glioma patients' data in the CGGA database were introduced to validate the effectiveness of the signatures among Chinese populations. Excluding the previously reported prognostic markers of gliomas from this study, the expression of HSPA5 and MTPN were examined by qRT-PCR and immunohistochemical assay. Results. In total, 20 mRNAs were finally selected to build PI for patients from TCGA, including 16 high-risk genes and four low-risk genes. For Chinese patients, the log-rank test p values of PI were both less than 0.0001 in two independent datasets. And the AUCs were 0.831 and 0.907 for 3 years of two datasets, respectively. Moreover, among these 20 mRNAs, 10 and 15 mRNAs also had a significant predictive effect via univariate COX analysis in CGGA_693 and CGGA_325, respectively. qRT-PCR and Immunohistochemistry assay indicated that HSPA5 and MTPN over-expressed in Glioma samples compared to normal samples. Conclusion. The 20-gene signature can forecast the risk of Glioma in TCGA effectively, moreover it can also predict the risks of Chinese patients through validation in the CGGA database. HSPA5 and MTPN are possible biomarkers of gliomas suitable for all populations to improve the prognosis of these patients. |
| WOS关键词 | GLIOBLASTOMA ; CLASSIFICATION ; INHIBITION ; BIOMARKER ; THERAPY ; REVEALS ; TUMORS ; ERK |
| 资助项目 | Fundamental Research Funds for the Central Universities[2019jbkyzy002] ; Fundamental Research Funds for the Central Universities[lzujbky-2019-ct02] ; Fundamental Research Funds for the Central Universities[2020jbkyzx001] ; Fundamental Research Funds for the Central Universities[lzujbky-2020-kb20] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000649824500006 |
| 出版者 | PEERJ INC |
| 资助机构 | Fundamental Research Funds for the Central Universities |
| 源URL | [http://119.78.100.186/handle/113462/137250]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Yang, Kehu |
| 作者单位 | 1.Lanzhou Univ, Dept Anesthesiol, Hosp 2, Lanzhou, Peoples R China 2.Lanzhou Univ, Evidence Based Med Ctr, Lanzhou, Peoples R China 3.Lanzhou Univ, Coll Pharm, Lanzhou, Peoples R China 4.Zhejiang Univ, Sch Med, Sch Publ Hlth, Hangzhou, Peoples R China 5.Zhejiang Univ, Sch Med, Affiliated Hosp 2, Hangzhou, Peoples R China 6.Chinese Acad Sci, Inst Modern Phys, Dept Computat Phys, Lanzhou, Peoples R China 7.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Lanzhou, Peoples R China 8.Lanzhou Univ, Dept Neurosurg, Hosp 2, Lanzhou, Peoples R China 9.Lanzhou Univ, Clin Med Coll 1, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jieting,Zhang, Hongrui,Zhang, Jingyun,et al. Identification of robust diagnostic and prognostic gene signatures in different grades of gliomas: a retrospective study[J]. PEERJ,2021,9:23. |
| APA | Liu, Jieting.,Zhang, Hongrui.,Zhang, Jingyun.,Bing, Zhitong.,Wang, Yingbin.,...&Yang, Kehu.(2021).Identification of robust diagnostic and prognostic gene signatures in different grades of gliomas: a retrospective study.PEERJ,9,23. |
| MLA | Liu, Jieting,et al."Identification of robust diagnostic and prognostic gene signatures in different grades of gliomas: a retrospective study".PEERJ 9(2021):23. |
入库方式： OAI收割
来源：近代物理研究所
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