Fluorescent Probes for Imaging Protein Disulfides in Live Organisms
文献类型：期刊论文
| 作者 | Hu, Guodong1,3; Zhong, Miao1,3; Zhao, Jintao1,3; Gao, Hao4; Gan, Lu2 ; Zhang, Hong2; Zhang, Shengxiang4; Fang, Jianguo1,3
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| 刊名 | ACS SENSORS
 |
| 出版日期 | 2021-03-26 |
| 卷号 | 6期号:3页码:1384-1391 |
| 关键词 | fluorescent probes environment-sensitive probes protein disulfides in vivo imaging two-photon mouse stroke model |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.1c00049 |
| 通讯作者 | Fang, Jianguo(fangjg@lzu.edu.cn) |
| 英文摘要 | Cellular redox homeostasis is predominantly controlled by the ratio of thiols and disulfides, and reversible thioldisulfide exchange reactions are fundamental of the biological redox regulation. However, due to the dynamic exchanges of thiols and disulfides, the detection, especially the in situ detection, of protein disulfides (PDS) is challenging. We employ the strategy, i.e., the increase of emission upon an environment-sensitive dye binding to proteins, to design PDS probes and discover a two-photon probe PDSTP590 (S6) that selectively recognizes PDS in live organisms. With the aid of the probe, we further disclose the elevation of PDS in brains of the mouse stroke model. |
| 资助项目 | National Natural Science Foundation of China[21778028] ; National Natural Science Foundation of China[22077055] ; Natural Science Foundation of Gansu Province[20JR5RA311] ; Natural Science Foundation of Gansu Province[18JR4RA003] ; 111 project |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000635484500086 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province ; 111 project |
| 源URL | [http://119.78.100.186/handle/113462/137604]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Fang, Jianguo |
| 作者单位 | 1.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Gansu Key Lab Biomonitoring & Bioremediat Environ, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Guodong,Zhong, Miao,Zhao, Jintao,et al. Fluorescent Probes for Imaging Protein Disulfides in Live Organisms[J]. ACS SENSORS,2021,6(3):1384-1391. |
| APA | Hu, Guodong.,Zhong, Miao.,Zhao, Jintao.,Gao, Hao.,Gan, Lu.,...&Fang, Jianguo.(2021).Fluorescent Probes for Imaging Protein Disulfides in Live Organisms.ACS SENSORS,6(3),1384-1391. |
| MLA | Hu, Guodong,et al."Fluorescent Probes for Imaging Protein Disulfides in Live Organisms".ACS SENSORS 6.3(2021):1384-1391. |
入库方式： OAI收割
来源：近代物理研究所
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