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Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress

文献类型：期刊论文

作者Gao, Yue3,4; Zhou, Xiang3,4; Zhang, Miao-Miao1,3,4; Liu, Ya-Jun2,4; Guo, Xiao-Peng5; Lei, Cai-Rong3,4; Li, Wen-Jian1,3,4; Lu, Dong1,3,4
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版日期2021-02-19
页码18
关键词Clostridium acetobutylicum Membrane characteristics Surface hydrophobicity Membrane fatty acids Membrane potential Genome-wide resequencing
ISSN号0175-7598
DOI10.1007/s00253-021-11174-5
通讯作者Lu, Dong(LD@impcas.ac.cn)
英文摘要Butanol inhibits bacterial activity by destroying the cell membrane of Clostridium acetobutylicum strains and altering functionality. Butanol toxicity also results in destruction of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS), thereby preventing glucose transport and phosphorylation and inhibiting transmembrane transport and assimilation of sugars, amino acids, and other nutrients. In this study, based on the addition of exogenous butanol, the tangible macro indicators of changes in the carbon ion beam irradiation-mutant Y217 morphology were observed using scanning electron microscopy (SEM). The mutant has lower microbial adhesion to hydrocarbon (MATH) value than C. acetobutylicum ATCC 824 strain. FDA fluorescence intensity and conductivity studies demonstrated the intrinsically low membrane permeability of the mutant membrane, with membrane potential remaining relatively stable. Monounsaturated FAs (MUFAs) accounted for 35.17% of the mutant membrane, and the saturated fatty acids (SFA)/unsaturated fatty acids (UFA) ratio in the mutant cell membrane was 1.65. In addition, we conducted DNA-level analysis of the mutant strain Y217. Expectedly, through screening, we found gene mutant sites encoding membrane-related functions in the mutant, including ATP-binding cassette (ABC) transporter-related genes, predicted membrane proteins, and the PTS transport system. It is noteworthy that an unreported predicted membrane protein (CAC 3309) may be related to changes in mutant cell membrane properties.
资助项目National Natural Science Foundation of China[11975284] ; National Natural Science Foundation of China[11905265] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-QYZD-197] ; Project of Lanzhou Science and Technology[2019-1-39]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000619755300001
出版者SPRINGER
资助机构National Natural Science Foundation of China ; Science and Technology Service Network Initiative of Chinese Academy of Sciences ; Project of Lanzhou Science and Technology
源URL[http://119.78.100.186/handle/113462/137918]  
专题中国科学院近代物理研究所
通讯作者Lu, Dong
作者单位1.Gansu Key Lab Microbial Resources Exploitat & App, Lanzhou 730070, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Yue,Zhou, Xiang,Zhang, Miao-Miao,et al. Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2021:18.
APA Gao, Yue.,Zhou, Xiang.,Zhang, Miao-Miao.,Liu, Ya-Jun.,Guo, Xiao-Peng.,...&Lu, Dong.(2021).Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,18.
MLA Gao, Yue,et al."Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2021):18.

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来源：近代物理研究所

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