Nanoscale insight into chromatin remodeling and DNA repair complex in HeLa cells after ionizing radiation
文献类型：期刊论文
| 作者 | Wu, Ruqun2,3 ; Liu, Wenjing2,3; Sun, Yujie1; Shen, Cheng2; Guo, Jinlong3; Zhao, Jing2; Mao, Guangbo2; Li, Yaning3; Du, Guanghua3
|
| 刊名 | DNA REPAIR
 |
| 出版日期 | 2020-12-01 |
| 卷号 | 96页码:7 |
| 关键词 | DNA repair Chromatin remodeling DNA double-strand break Single molecule localization microscopy Ionizing radiation |
| ISSN号 | 1568-7864 |
| DOI | 10.1016/j.dnarep.2020.102974 |
| 通讯作者 | Du, Guanghua(gh_du@impcas.ac.cn) |
| 英文摘要 | The dynamic structure of nuclear chromatin and its regulation in the formation of repair complex is essential in DNA damage response and repair. Using single molecule localization microscopy STORM this work discovered that the nuclear chromatin organization was relaxed from 200 to 400 nm thick irregular frame and remodeled to dispersed sub-100 nm structure in HeLa cells after X-ray irradiation. The DSB repair factors (gamma-H2AX, MDC1, 53BP1) showed distribution as microscale-colocalized and nanoscale interlaced substructure in the DSB repair complex. The dual-color nanoscopic imaging of gamma-H2AX and chromatin at the DSB sites suggest that DNA damage response and repair cascade are chromatin structure-dependent and also partly dependent on the distance to the DSB sites. The sub-100 nm structure of fibers and nanoclusters of the relaxed nuclear chromatin and the DSB repair factors highly resembled the cross-section view of chromatin organization. These data demonstrated the polymorphic and dynamic behavior of the chromatin organization in vivo, and provided nanoscale insight into the interplay between chromatin remodeling and DNA damage response and DNA repair. |
| WOS关键词 | HISTONE H2AX PHOSPHORYLATION ; GAMMA-H2AX ; ORGANIZATION ; RECRUITMENT ; DYNAMICS ; BREAKS ; FOCI |
| 资助项目 | National Natural Science Foundation of China[U1632271] ; National Natural Science Foundation of China[11975283] |
| WOS研究方向 | Genetics & Heredity ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000591723400003 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/139452]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Du, Guanghua |
| 作者单位 | 1.Peking Univ, BIOPIC, Beijing 100871, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Ruqun,Liu, Wenjing,Sun, Yujie,et al. Nanoscale insight into chromatin remodeling and DNA repair complex in HeLa cells after ionizing radiation[J]. DNA REPAIR,2020,96:7. |
| APA | Wu, Ruqun.,Liu, Wenjing.,Sun, Yujie.,Shen, Cheng.,Guo, Jinlong.,...&Du, Guanghua.(2020).Nanoscale insight into chromatin remodeling and DNA repair complex in HeLa cells after ionizing radiation.DNA REPAIR,96,7. |
| MLA | Wu, Ruqun,et al."Nanoscale insight into chromatin remodeling and DNA repair complex in HeLa cells after ionizing radiation".DNA REPAIR 96(2020):7. |
入库方式： OAI收割
来源：近代物理研究所
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