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Integrated analysis of circRNA-miRNA-mRNA network reveals potential prognostic biomarkers for radiotherapies with X-rays and carbon ions in non-small cell lung cancer

文献类型：期刊论文

作者Jin, Xiaodong1,3,4,5; Yuan, Lingyan1,3; Liu, Bingtao1,3,4,5; Kuang, Yanbei1,3,4,5; Li, Hongbin1,3,4,5; Li, Linying1,3,4,5; Zhao, Xueshan2; Li, Feifei1,3,4,5; Bing, Zhitong1,3; Chen, Weiqiang1,3,4,5
刊名ANNALS OF TRANSLATIONAL MEDICINE
出版日期2020-11-01
卷号8期号:21页码:12
关键词Carbon-ion radiotherapy (CIRT) radiosensitivity circRNA-miRNA-mRNA network non-small cell lung cancer (NSCLC)
ISSN号2305-5839
DOI10.21037/atm-20-2002
通讯作者Li, Qiang(liqiang@impcas.ac.cn)
英文摘要Background: This work was aimed at exploring the regulatory network of non-coding RNA (ncRNA) especially circular RNA (circRNA) and microRNA (miRNA), in the sensitivity of non-small cell lung cancer (NSCLC) cells to low linear energy transfer (LET) X-ray and high-LET carbon ion irradiations. Methods: The radioresistant NSCLC cell line A549-R11 was obtained from its parental cell line A549 through irradiation with X-rays of 2.0 Gy per fraction for 30 times. The sensitivities of A549, A549-R11 and H1299 cells exposed to X-rays and carbon ions were verified using the colony formation assay. A comprehensive circRNA-miRNA-mRNA network was constructed through the sequencing data in parental A549, acquired radioresistant A549-R11 and intrinsic radioresistant H1299 cells, and the network was further optimized according to the prognostic results from the TCGA and GEO databases. Results: Based on high-throughput sequencing of circRNAs, we found that 40 circRNAs were up -regulated while 184 circRNAs were down-regulated in the intersection of the sets of A549-R11 and H1299 cells. Subsequently, a circRNA-miRNA-mRNA network, including 14 interactive pairs and 8 circRNAs, 4 overall survival-associated miRNAs, and 4 mRNAs, was constructed through the high-throughput data screening and bioinformatics methods. Conclusions: Our results provide a complete understanding to the regulatory mechanism of the sensitivities to low-LET X-ray and high-LET carbon ion irradiations, and might be helpful to screen potential biomarkers for predicting the Carbon-ion radiotherapy (CIRT) and X-ray radiotherapy responses in NSCLC.
WOS关键词CIRCULAR RNA ; RADIATION-THERAPY ; EXPRESSION ; RADIORESISTANCE ; CARCINOMA ; APOPTOSIS ; ARREST
资助项目National Key Research and Development Program of China[2018YFC0115702] ; Key Deployment Project of Chinese Academy of Sciences[KFZDSW-222] ; National Natural Science Foundation of China[11705245] ; National Natural Science Foundation of China[11875299] ; National Natural Science Foundation of China[U1532264] ; National Natural Science Foundation of China[U1730133]
WOS研究方向Oncology ; Research & Experimental Medicine
语种英语
WOS记录号WOS:000590129400007
出版者AME PUBL CO
资助机构National Key Research and Development Program of China ; Key Deployment Project of Chinese Academy of Sciences ; National Natural Science Foundation of China
源URL[http://119.78.100.186/handle/113462/139476]  
专题中国科学院近代物理研究所
通讯作者Li, Qiang
作者单位1.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing, Peoples R China
2.Lanzhou Univ, Dept Oncol Radiotherapy, First Hosp, Lanzhou, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Peoples R China
4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China
5.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Jin, Xiaodong,Yuan, Lingyan,Liu, Bingtao,et al. Integrated analysis of circRNA-miRNA-mRNA network reveals potential prognostic biomarkers for radiotherapies with X-rays and carbon ions in non-small cell lung cancer[J]. ANNALS OF TRANSLATIONAL MEDICINE,2020,8(21):12.
APA Jin, Xiaodong.,Yuan, Lingyan.,Liu, Bingtao.,Kuang, Yanbei.,Li, Hongbin.,...&Li, Qiang.(2020).Integrated analysis of circRNA-miRNA-mRNA network reveals potential prognostic biomarkers for radiotherapies with X-rays and carbon ions in non-small cell lung cancer.ANNALS OF TRANSLATIONAL MEDICINE,8(21),12.
MLA Jin, Xiaodong,et al."Integrated analysis of circRNA-miRNA-mRNA network reveals potential prognostic biomarkers for radiotherapies with X-rays and carbon ions in non-small cell lung cancer".ANNALS OF TRANSLATIONAL MEDICINE 8.21(2020):12.

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来源：近代物理研究所

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