Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell
文献类型：期刊论文
| 作者 | Ma, Jianlong2,3,4; Lu, Xiaofeng2,3; Zhai, Honglin1; Li, Qiang5 ; Qiao, Li6; Guo, Yong2,3
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| 刊名 | TALANTA
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| 出版日期 | 2020-11-01 |
| 卷号 | 219页码:10 |
| 关键词 | Fluorescent probe Rational design Highly selective Butyrylcholinesterase |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2020.121278 |
| 通讯作者 | Qiao, Li(qiaolisurgeon@126.com) ; Guo, Yong(guoyong@licp.cas.cn) |
| 英文摘要 | In the current work, a near-infrared (NIR) fluorescent probe (CyClCP) was developed for fast (35 min), highly sensitive (LOD of 3.75 U/L) and selective response to BChE in vitro and in vivo. Upon the addition of BChE, CyClCP could be efficiently activated with remarkable NIR (lambda(em) = 708 nm) fluorescence enhancement and obvious absorbance red shift (581 nm-687 nm). Specifically, according to the subtle differences structural features and substrate preference between BChE and its sister enzyme AChE, CyClCP was constructed by introducing chlorine atom at the ortho-position of the phenolic hydroxyl in the previous reported probe (CyCP). Fortunately, CyClCP exhibited better selectivity towards BChE over AChE compared with CyCP. This molecular design strategy was further rationalized by docking molecular of fluorescence probes (CyClCP and CyCP) and enzymes (BChE and AChE). Finally, CyClCP was membrane permeable and successfully applied to image endogenous BChE level in HepG2 and LO2 cells. Therefore, CyClCP could serve as a promising tool for BChErelated physiological function studies in complex biological systems. |
| WOS关键词 | COCAINE HYDROLYSIS ; CRYSTAL-STRUCTURE ; ACETYLCHOLINESTERASE ; STRATEGY ; INHIBITION ; MECHANISM ; DISCOVERY ; SUBSTRATE ; VARIANTS ; HISTORY |
| 资助项目 | National Natural Science Foundation of China[21575149] ; National Natural Science Foundation of China[21505146] ; National Natural Science Foundation of China[21575148] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000568752900008 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/139601]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Qiao, Li; Guo, Yong |
| 作者单位 | 1.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 6.Gansu Prov Hosp, Dept Neurosurg, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Jianlong,Lu, Xiaofeng,Zhai, Honglin,et al. Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell[J]. TALANTA,2020,219:10. |
| APA | Ma, Jianlong,Lu, Xiaofeng,Zhai, Honglin,Li, Qiang,Qiao, Li,&Guo, Yong.(2020).Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell.TALANTA,219,10. |
| MLA | Ma, Jianlong,et al."Rational design of a near-infrared fluorescence probe for highly selective sensing butyrylcholinesterase (BChE) and its bioimaging applications in living cell".TALANTA 219(2020):10. |
入库方式： OAI收割
来源：近代物理研究所
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