Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice
文献类型：期刊论文
| 作者 | Liu, Cuihua1; Tanaka, Kaoru1; Katsube, Takanori1; Vares, Guillaume2; Maruyama, Kouichi1; Ninomiya, Yasuharu1; Fardous, Zeenath3; Sun, Chao4 ; Fujimori, Akira1; Moreno, Stephanie G.5
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| 刊名 | DOSE-RESPONSE
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| 出版日期 | 2020-07-01 |
| 卷号 | 18期号:3页码:11 |
| 关键词 | green fluorescent protein apoptosis bone marrow death adaptive response ionizing radiation GFP transgenic mice |
| ISSN号 | 1559-3258 |
| DOI | 10.1177/1559325820951332 |
| 通讯作者 | Nenoi, Mitsuru(nenoi.mitsuru@qst.go.jp) ; Wang, Bing(wang.bing@qst.go.jp) |
| 英文摘要 | Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice. |
| WOS关键词 | GREEN FLUORESCENT PROTEIN ; TRANSPLANTED PANCREATIC-ISLETS ; MARROW-DERIVED CELLS ; OXIDATIVE STRESS ; IN-VIVO ; RADIOADAPTIVE RESPONSE ; TRANSGENIC EXPRESSION ; ADAPTIVE RESPONSE ; GERM-CELLS ; GFP |
| 资助项目 | Ministry of Education, Culture, Sport, Sciences and Technology (MEXT), Japan ; Nuclear Safety Research Association, Japan ; JSPS KAKENHI, Japan[25340041] |
| WOS研究方向 | Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000566468700001 |
| 出版者 | SAGE PUBLICATIONS INC |
| 资助机构 | Ministry of Education, Culture, Sport, Sciences and Technology (MEXT), Japan ; Nuclear Safety Research Association, Japan ; JSPS KAKENHI, Japan |
| 源URL | [http://119.78.100.186/handle/113462/139804]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Nenoi, Mitsuru; Wang, Bing |
| 作者单位 | 1.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Chiba, Japan 2.Okinawa Inst Sci & Technol, Cell Signal Unit, Okinawa, Japan 3.Bangladesh Atom Energy Commiss, Inst Food & Radiat Biol, Atom Energy Res Estab, Dhaka, Bangladesh 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 5.INSERM, Fundamental Res Div, LRTS Francois Jacob Inst Biol, Atom Energy & Alternat Energies Commiss, Fontenay Aux Roses, France 6.Natl Inst Quantum & Radiol Sci & Technol, Dept Safety Adm, Chiba, Japan |
| 推荐引用方式 GB/T 7714 | Liu, Cuihua,Tanaka, Kaoru,Katsube, Takanori,et al. Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice[J]. DOSE-RESPONSE,2020,18(3):11. |
| APA | Liu, Cuihua.,Tanaka, Kaoru.,Katsube, Takanori.,Vares, Guillaume.,Maruyama, Kouichi.,...&Wang, Bing.(2020).Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice.DOSE-RESPONSE,18(3),11. |
| MLA | Liu, Cuihua,et al."Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice".DOSE-RESPONSE 18.3(2020):11. |
入库方式： OAI收割
来源：近代物理研究所
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