Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect
文献类型：期刊论文
| 作者 | Jia, Rong1,2,3; Chen, Yaxiong1,2 ; Jia, Cong4; Hu, Burong1,2,5; Du, Yarong1,2,4
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| 刊名 | INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
 |
| 出版日期 | 2020-08-19 |
| 页码 | 9 |
| 关键词 | MAVS irradiation ROS RIBE |
| ISSN号 | 0955-3002 |
| DOI | 10.1080/09553002.2020.1807642 |
| 通讯作者 | Du, Yarong(duyrlive@impcas.ac.cn) |
| 英文摘要 | Purpose Mitochondrial antiviral signaling (MAVS) protein, located in the mitochondrial out-membrane, is necessary for IFN-beta induction and IFN-stimulated gene expression in response to external stress such as viral invasion and ionizing radiation (IR). Although the involvement of radiation induced bystander effect (RIBE) has been investigated for decades for secondary cancer risk related to radiotherapy, the underlying regulatory mechanisms remain largely unclear, especially the roles played by the immune factors such as MAVS. Material and methods MAVS gene knockout cells using CRISPR/Cas9 technology were used as donor cells or recipient cells to assess the role of MAVS in RIBE by means of co-cultured system. The micronucleus and gamma H2AX foci in the recipient cells were counted to demonstrate the degree of RIBE. The reactive oxygen species (ROS) level in the recipient was measured using the fluorescent dye 2 ' 7 '-dichlorofluorescein. Results Firstly, we found that MAVS expression level was different in A549, BEAS-2B, U937 and HepG2 cells. Cell co-culture experiments showed that MAVS participate in RIBE. Interestingly, the RIBE response was more significant in recipient cells with higher level of MAVS (i.e. A549) than that in recipient cells showing lower level of MAVS (i.e. BEAS-2B). Further, the bystander response was dramatically suppressed in MAVS-silenced A549 and BEAS-2B recipient cells. MAVS-silenced recipient cells exhibited lower level of ROS induced by IR. Conclusions Our results indicated that the innate immune signaling molecule MAVS in recipient cells participate in RIBE. ROS is an important factor in RIBE via MAVS pathway and MAVS may be a potential target for the precise radiotherapy and radioprotection. |
| WOS关键词 | OXIDATIVE STRESS ; DYNAMICS |
| 资助项目 | National Natural Science Foundation of China[11705248] ; National Natural Science Foundation of China[U1832101] ; National Natural Science Foundation of China[11635013] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; Science and Technology Research Project of Gansu Province[17JR5RA307] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000561846400001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | National Natural Science Foundation of China ; Science and Technology Research Project of Gansu Province |
| 源URL | [http://119.78.100.186/handle/113462/139869]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Du, Yarong |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 2.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Inst Modern Phys, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Inst Modern Phys, Beijing, Peoples R China 4.Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Rong,Chen, Yaxiong,Jia, Cong,et al. Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2020:9. |
| APA | Jia, Rong,Chen, Yaxiong,Jia, Cong,Hu, Burong,&Du, Yarong.(2020).Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,9. |
| MLA | Jia, Rong,et al."Suppression of innate immune signaling molecule, MAVS, reduces radiation-induced bystander effect".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2020):9. |
入库方式： OAI收割
来源：近代物理研究所
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