Macrophage-Mediated Bystander Effects after Different Irradiations through a p53-dependent Pathway
文献类型：期刊论文
| 作者 | Fu, Jiamei1,2; Zhu, Lin2; Tu, Wenzhi2; Wang, Xiangdong2; Pan, Yan2; Bai, Yang2; Dang, Bingrong3 ; Chen, Jiayi4; Shao, Chunlin2
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| 刊名 | RADIATION RESEARCH
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 193期号:2页码:119-129 |
| ISSN号 | 0033-7587 |
| DOI | 10.1667/RR15354.1 |
| 通讯作者 | Shao, Chunlin(clshao@shmu.edu.cn) |
| 英文摘要 | The goal of this work was to elucidate the mechanisms of bystander effects outside the localized irradiation field and their potential hematological toxicity. In this study, an in vitro multicellular co-culture system was used to investigate the intercellular commutation and related signaling pathways between either irradiated A549 cells or Beas-2B cells and bystander lymphoblast TK6 cells with or without macrophage U937 cells as an intermediator. Results showed that the proliferation ability of bystander TK6 cells was inhibited after co-culture with A549 cells irradiated with gamma rays rather than carbon ions. When macrophages were contained in the co-culture system, the cell viability damage to the bystander TK6 cells were further enhanced. However, the proliferation inhibition of bystander TK6 cells after co-culture with irradiated Bcas-2B cells was observed only when intermediator macrophages existed in the cell co-culture system. More serious cell injury was detected after carbon-ion irradiation compared with gamma-ray irradiation. The p53-relevant apoptosis pathway was activated in both irradiated A549 and Beas-2B cells, each to a different extent. When the p53 pathway of irradiated cells was inhibited by PFT-alpha, PFT-mu or p53 siRNA, the bystander damage to TK6 cells were clearly alleviated. In conclusion, the bystander lymphoblast damage was induced in different cells using different LET radiations. An amplified bystander response was modulated by the intermediator macrophage. The underlying molecular mechanisms of these bystander effects were dependent on the activation of p53 and its relevant apoptosis pathway in the irradiated cells. These results suggest that the bystander and macrophage-mediated bystander effects contribute to the common acute side effect of lymphocytopenia after local irradiation. (C) 2020 by Radiation Research Society |
| WOS关键词 | RADIATION-INDUCED BYSTANDER ; HEAVY-ION RADIATION ; PROTECTS MICE ; NORMAL TISSUE ; GAMMA-RAY ; P53 ; MECHANISMS ; APOPTOSIS ; LYMPHOPENIA ; EXPRESSION |
| 资助项目 | National Key R&D Program of China[2017YFC0108604] ; NSFC[31770910] ; NSFC[31570850] ; HIRFL-CSR Project (Lanzhou, China) |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| WOS记录号 | WOS:000518792700003 |
| 出版者 | RADIATION RESEARCH SOC |
| 资助机构 | National Key R&D Program of China ; NSFC ; HIRFL-CSR Project (Lanzhou, China) |
| 源URL | [http://119.78.100.186/handle/113462/140957]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Shao, Chunlin |
| 作者单位 | 1.Tongji Univ, Shanghai Pulm Hosp, Sch Med, Dept Radiat Oncol, Shanghai 200433, Peoples R China 2.Fudan Univ, Inst Radiat Med, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 4.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Radiat Oncol, Sch Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Jiamei,Zhu, Lin,Tu, Wenzhi,et al. Macrophage-Mediated Bystander Effects after Different Irradiations through a p53-dependent Pathway[J]. RADIATION RESEARCH,2020,193(2):119-129. |
| APA | Fu, Jiamei.,Zhu, Lin.,Tu, Wenzhi.,Wang, Xiangdong.,Pan, Yan.,...&Shao, Chunlin.(2020).Macrophage-Mediated Bystander Effects after Different Irradiations through a p53-dependent Pathway.RADIATION RESEARCH,193(2),119-129. |
| MLA | Fu, Jiamei,et al."Macrophage-Mediated Bystander Effects after Different Irradiations through a p53-dependent Pathway".RADIATION RESEARCH 193.2(2020):119-129. |
入库方式： OAI收割
来源：近代物理研究所
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