Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway
文献类型：期刊论文
| 作者 | Lu, Lina1,2; Li, Wenjun3,4; Sun, Chao5 ; Kang, Shuhe3; Li, Jia3; Luo, Xingping3; Su, Qiong3; Liu, Bin1,6; Qin, Song2,4
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| 刊名 | JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
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| 出版日期 | 2019-11-25 |
| 页码 | 10 |
| 关键词 | gut microbiota intestinal injury phycocyanin radiation |
| ISSN号 | 0148-6071 |
| DOI | 10.1002/jpen.1744 |
| 通讯作者 | Liu, Bin(binliu0736@126.com) |
| 英文摘要 | Background Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment. No safe and effective radiation countermeasure has been approved for clinical therapy. In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury. Materials and Methods C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy. The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor kappa B (NF-kappa B) signaling were evaluated. Results Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation. Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1). PC pretreatment significantly ameliorated radiation-induced intestinal injury. PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-kappa B pathway activation. Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, were also downregulated. Conclusion PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-kappa B pathway, suggesting PC as a promising natural radiation countermeasure. |
| WOS关键词 | INFLAMMATORY-BOWEL-DISEASE ; C-PHYCOCYANIN ; FECAL MICROBIOTA ; MOUSE ; PROTECTS ; INJURY |
| 资助项目 | National Key Research and Development Program of China[2018YFD0901102] ; Key Research and Development Program of Yantai[Y933021011] ; National Natural Science Foundation of China[11775280] ; Northwest Minzu University's Double First-class and Characteristic Development Guide Special Funds-Chemistry Key Disciplines in Gansu Province[11080316] ; Fundamental Research Funds for the Central Universities[31920180020] ; Fundamental Research Funds for the Central Universities[31920190013] ; Fundamental Research Funds for the Central Universities[31920190018] |
| WOS研究方向 | Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000498400500001 |
| 出版者 | WILEY |
| 资助机构 | National Key Research and Development Program of China ; Key Research and Development Program of Yantai ; National Natural Science Foundation of China ; Northwest Minzu University's Double First-class and Characteristic Development Guide Special Funds-Chemistry Key Disciplines in Gansu Province ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://119.78.100.186/handle/113462/141218]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Liu, Bin |
| 作者单位 | 1.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou, Gansu, Peoples R China 2.Northwest Minzu Univ, Sch Chem Engn, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Biol & Bioresource Utilizat, Yantai, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China 6.Lanzhou Univ, Sch Stomatol, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Lina,Li, Wenjun,Sun, Chao,et al. Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway[J]. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION,2019:10. |
| APA | Lu, Lina.,Li, Wenjun.,Sun, Chao.,Kang, Shuhe.,Li, Jia.,...&Qin, Song.(2019).Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway.JOURNAL OF PARENTERAL AND ENTERAL NUTRITION,10. |
| MLA | Lu, Lina,et al."Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-kappa B Pathway".JOURNAL OF PARENTERAL AND ENTERAL NUTRITION (2019):10. |
入库方式： OAI收割
来源：近代物理研究所
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