An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells
文献类型：期刊论文
| 作者 | Wang, Chun-Ming1; Huo, Xiang1; Chen, Jun1; Liu, Jia-Wei1; Yang, Ting-Yu1; Mi, Xiang-Quan1; Meng, Yue1; Zhou, Li1; Lin, Chang-Jun1; Liu, Jing2
|
| 刊名 | TOXICOLOGY IN VITRO
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 62页码:7 |
| 关键词 | Xanthohumol Reactive oxygen species Pro-oxidant Acute lytic cell death Cell rupture |
| ISSN号 | 0887-2333 |
| DOI | 10.1016/j.tiv.2019.104667 |
| 通讯作者 | Wang, Chun-Ming(cmwang@lzu.edu.cn) ; Liu, Jing(liuj@impcas.ac.cn) |
| 英文摘要 | Serum is an important component in cell culture medium. It also possesses potent antioxidant properties. Therefore, the conventional protocols for detecting reactive oxygen species (ROS) in cultured cells with fluorescent probes include washing and suspending cells with serum-free buffers, such as PBS. This transient serum deprivation is essential for the ROS detecting. Unfortunately, it may also cause unexpected results, which push us to choose more optimal experiment conditions. In the present study, we found an acute lytic cell death induced by xanthohumol (XN), which obstructed ROS detecting in human leukemia cell line HL-60 cells. XN induced ROS burst, caused cell swelling, membrane permeability increase, LDH release, and ultimately an acute lyric cell death and cell rupture. These effects could be alleviated by the antioxidant N-Acetyl-L-cysteine (NAC). Apoptosis, pyroptosis or necroptosis were not observed in this process. Results also indicated that 2% serum addition had already completely scavenged ROS induced by 10 mu M XN. Taken together, it is strongly suggested to detecting ROS in a serum-free medium when studying where and how ROS generated in cells. The concentration at the ROS maximum point (10 mu M XN in this study) can be selected as the optimal concentration. |
| WOS关键词 | PHARMACOLOGICAL PROFILE ; ANTIOXIDANT PROPERTIES ; APOPTOSIS ; GENERATION ; HOPS |
| 资助项目 | National Natural Science Foundation of China[31071917] ; Western Light Foundation ; Technology to support Gansu Province Foundation of Chinese Academy of Sciences[KJZG-2011-002] |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000500376200001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China ; Western Light Foundation ; Technology to support Gansu Province Foundation of Chinese Academy of Sciences |
| 源URL | [http://119.78.100.186/handle/113462/141241]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Wang, Chun-Ming; Liu, Jing |
| 作者单位 | 1.Lanzhou Univ, Sch Life Sci, Minist Educ, Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chun-Ming,Huo, Xiang,Chen, Jun,et al. An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells[J]. TOXICOLOGY IN VITRO,2020,62:7. |
| APA | Wang, Chun-Ming.,Huo, Xiang.,Chen, Jun.,Liu, Jia-Wei.,Yang, Ting-Yu.,...&Liu, Jing.(2020).An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells.TOXICOLOGY IN VITRO,62,7. |
| MLA | Wang, Chun-Ming,et al."An acute lytic cell death induced by xanthohumol obstructed ROS detecting in HL-60 cells".TOXICOLOGY IN VITRO 62(2020):7. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。