Genome-Wide Comparisons of Mutations Induced by Carbon-Ion Beam and Gamma-Rays Irradiation in Rice via Resequencing Multiple Mutants
文献类型：期刊论文
| 作者 | Yang, Guili1; Luo, Wenlong1; Zhang, Jian1; Yan, Xiancheng1; Du, Yan2 ; Zhou, Libin2; Li, Wenjian2; Wang, Hui1; Chen, Zhiqiang1; Guo, Tao1
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| 刊名 | FRONTIERS IN PLANT SCIENCE
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| 出版日期 | 2019-11-28 |
| 卷号 | 10页码:13 |
| 关键词 | Oryza sativa L carbon-ion beams (CIBs) irradiation gamma rays (GRs) resequencing single base substitution (SBS) InDels multiple nucleotide variant (MNV) structural variant (SV) |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2019.01514 |
| 通讯作者 | Guo, Tao(guo.tao@vip.163.com) |
| 英文摘要 | Physical mutagens, such as carbon-ion beams (CIBs) and gamma rays (GRs), induce mutations with high frequency at a relatively low dose and are more user-friendly and environment-friendly in mutation breeding. Previous studies showed that CIBs induced large sized deletions and insertions, and chromosomal rearrangements, whereas GRs induce shorter deletions and insertions, and more frequent base substitutions. However, the difference on the genomic level between CIB- and GR-induced mutations remains to be clarified. In the present study, we re-sequence six mutagenized lines derived from CIB irradiation and four mutagenized lines derived from GRs. A total of 283 and 381 variations are induced in these mutants by CIBs and GRs, respectively, including single base substitutions (SBSs), small insertion and deletions (InDels), multiple nucleotide variants (MNVs). SBSs are the most abundant type of mutation and single base transition is the main form for SBSs. CIB-induced InDels accounted for 25.44% of the total variations, while GR-induced InDels accounted for 17.85%. On the contrary, the frequency of MNVs induced by GRs was approximately 2.19 times that induced by CIBs, which indicates CIBs induced increased InDels, whereas GRs induced increased MNVs. Notably, multiple base deletions (>= 5 bp) were induced at a much higher frequency by CIBs than by GRs. We also find mutations induced by CIBs and GRs are unevenly distributed on chromosomes. Unusual high-frequency (HF) and low-frequency (LF) mutation regions are discovered by analyzing mutations per 1Mb along the genome. The mutation frequency within the HF regions were significantly higher than the LF regions (P < 0.05). A large majority of SBSs, InDels, and MNVs induced by CIBs and GRs occurred in upstream and downstream regions. Our study compares difference of mutation profiles induced by the CIB irradiation and GR on rice genomes, and give some clues for understanding the mutagenesis mechanism of physical radiation and improving the mutagenesis efficiency. |
| WOS关键词 | DOUBLE-STRAND BREAKS ; LINEAR-ENERGY-TRANSFER ; DNA-DAMAGE ; MAMMALIAN-CELLS ; RADIATION ; IDENTIFICATION ; LESIONS ; INDUCTION ; DISCOVERY ; SPECTRUM |
| 资助项目 | National Key Technology Research and Development Program of China[2016YFD0102102] ; key Research and development Project of Guangdong Province[2018B020206002] ; Modern Agro-Industry Technology Research System[CARS-01-12] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000502355900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Key Technology Research and Development Program of China ; key Research and development Project of Guangdong Province ; Modern Agro-Industry Technology Research System |
| 源URL | [http://119.78.100.186/handle/113462/141262]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Guo, Tao |
| 作者单位 | 1.South China Agr Univ, Natl Engn Res Ctr Plant Space Breeding, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Guili,Luo, Wenlong,Zhang, Jian,et al. Genome-Wide Comparisons of Mutations Induced by Carbon-Ion Beam and Gamma-Rays Irradiation in Rice via Resequencing Multiple Mutants[J]. FRONTIERS IN PLANT SCIENCE,2019,10:13. |
| APA | Yang, Guili.,Luo, Wenlong.,Zhang, Jian.,Yan, Xiancheng.,Du, Yan.,...&Guo, Tao.(2019).Genome-Wide Comparisons of Mutations Induced by Carbon-Ion Beam and Gamma-Rays Irradiation in Rice via Resequencing Multiple Mutants.FRONTIERS IN PLANT SCIENCE,10,13. |
| MLA | Yang, Guili,et al."Genome-Wide Comparisons of Mutations Induced by Carbon-Ion Beam and Gamma-Rays Irradiation in Rice via Resequencing Multiple Mutants".FRONTIERS IN PLANT SCIENCE 10(2019):13. |
入库方式： OAI收割
来源：近代物理研究所
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