Quantitative multi-omics analysis of the effects of mitochondrial dysfunction on lipid metabolism in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Guo, Xiaopeng1,2; Zhang, Miaomiao1,2,3 ; Gao, Yue1,2; Cao, Guozhen4; Lu, Dong1,3; Li, Wenjian1,3
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| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 出版日期 | 2020-02-01 |
| 卷号 | 104期号:3页码:1211-1226 |
| ISSN号 | 0175-7598 |
| 关键词 | Multi-omics analysis Mitochondria Lipid metabolism Respiration-deficient mutant Saccharomyces cerevisiae |
| DOI | 10.1007/s00253-019-10260-z |
| 通讯作者 | Lu, Dong(ld@impcas.ac.cn) |
| 英文摘要 | In this study, combined genome, transcriptome, and metabolome analysis was performed for eight Saccharomyces cerevisiae mitochondrial respiration-deficient mutants. Each mutant exhibited a unique nuclear genome mutation pattern; the nuclear genome mutations, and thus potentially affected genes and metabolic pathways, showed a co-occurrence frequency of <= 3 among the eight mutants. For example, only a lipid metabolism-related pathway was likely to be affected by the nuclear genome mutations in one of the mutants. However, large deletions in the mitochondrial genome were the shared characteristic among the eight mutants. At the transcriptomic level, lipid metabolism was the most significantly enriched Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway for differentially expressed genes (DEGs) co-occurring in both >= 4 and >= 5 mutants. Any identified DEG enriched in lipid metabolism showed the same up-/down-regulated pattern among nearly all eight mutants. Further, 126 differentially expressed lipid species (DELS) were identified, which also showed the same up-/down-regulated pattern among nearly all investigated mutants. It was conservatively demonstrated that the similar change pattern of lipid metabolism in the entire investigated mutant population was attributed to mitochondrial dysfunction. The change spectrum of lipid species was presented, suggesting that the number and change degree of up-regulated lipid species were higher than those of down-regulated lipid species. Additionally, energy storage lipids increased in content and plasma-membrane phospholipid compositions varied in the relative proposition. The results for the genome, transcriptome, and lipidome were mutually validated, which provides quantitative data revealing the roles of mitochondria from a global cellular perspective. |
| WOS关键词 | DNA-DAMAGE ; PHOSPHATIDYLETHANOLAMINE ; MEMBRANE ; ACCUMULATION ; ETHANOLAMINE ; MUTATIONS ; AUTOPHAGY ; HEALTH |
| 资助项目 | National Natural Science Foundation of China[11975284] ; National Natural Science Foundation of China[11905265] ; Chinese Academy of Sciences[CAS-ITRI 2019012] ; Science and Technology Program of Lanzhou, China[2019-1-39] ; Industrial Technology Research Institute[CAS-ITRI 2019012] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000509529900024 |
| 资助机构 | National Natural Science Foundation of China ; Chinese Academy of Sciences ; Science and Technology Program of Lanzhou, China ; Industrial Technology Research Institute |
| 源URL | [http://119.78.100.186/handle/113462/141479]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Lu, Dong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Gansu Key Lab Microbial Resources Exploitat & App, Lanzhou 730000, Peoples R China 4.Univ Sci & Technol China, Hefei 230026, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiaopeng,Zhang, Miaomiao,Gao, Yue,et al. Quantitative multi-omics analysis of the effects of mitochondrial dysfunction on lipid metabolism in Saccharomyces cerevisiae[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(3):1211-1226. |
| APA | Guo, Xiaopeng,Zhang, Miaomiao,Gao, Yue,Cao, Guozhen,Lu, Dong,&Li, Wenjian.(2020).Quantitative multi-omics analysis of the effects of mitochondrial dysfunction on lipid metabolism in Saccharomyces cerevisiae.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(3),1211-1226. |
| MLA | Guo, Xiaopeng,et al."Quantitative multi-omics analysis of the effects of mitochondrial dysfunction on lipid metabolism in Saccharomyces cerevisiae".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.3(2020):1211-1226. |
入库方式： OAI收割
来源：近代物理研究所
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