The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b
文献类型：期刊论文
| 作者 | Ding, Nan1,2 ; Hua, Junrui1,2; He, Jinpeng1,2; Lu, Dong1,2; Wei, Wenjun1,2; Zhang, Yanan1,2; Zhou, Heng1,2; Zhang, Liying3; Liu, Yongqi3; Zhou, Guangming4
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| 刊名 | JOURNAL OF CANCER
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| 出版日期 | 2020 |
| 卷号 | 11期号:8页码:2222-2233 |
| 关键词 | microRNA radiation miR-5094 STAT5b proliferation |
| ISSN号 | 1837-9664 |
| DOI | 10.7150/jca.39679 |
| 通讯作者 | Wang, Jufang(jufangwang@impcas.ac.cn) |
| 英文摘要 | MicroRNAs (miRNAs) play important roles in the regulation of cellular stress responses. We previously uncovered 10 novel human miRNAs which are induced by X-ray irradiation in HeLa cells using Solexa deep sequencing. The most highly expressed new miRNA, miR-5094, was predicted to target STAT5b. This study wonders whether miR-5094 participates in cellular radiation response via STAT5b. Firstly, direct interaction between miRNA-5094 and the STAT5b 3'-UTR was confirmed by luciferase reporter assay. Then, the radiation responsive expression of miR-5094 and STAT5b were measured in HeLa and Jurkat cells, and the expressions of down-stream genes of STAT5b after ionizing radiation (IR) were detected in HeLa cells. At last, the effects of miR-5094 on survival fraction, cell proliferation, cell cycle arrest and apoptosis induced by IR were investigated in HeLa cells, Jurkat cells and human peripheral blood T cells. It was found that up-regulation of miR-5094 by radiation induction or miRNA mimic transfection suppressed expression of STAT5b, and consequently decreased the transcription of down-stream Igf-1 and Bcl-2. Additionally, over expression of miR-5094 resulted in proliferation suppression and knockdown of miR-5094 by miRNA inhibitor after irradiation partially reversed the proliferation suppression induced by miR-5094 in HeLa cells, Jurkat cells and CD4(+) T cells. Collectively, our findings demonstrate that up-regulation of miR-5094 down-regulated the expression of STAT5b, thereby suppressing cell proliferation after X-ray irradiation. |
| WOS关键词 | T-CELLS ; MICRORNAS ; EXPRESSION ; DEFICIENCY ; GROWTH ; IDENTIFICATION ; IRRADIATION ; ACTIVATION ; PROTEINS |
| 资助项目 | National Key R&D program of China[2017YFC0 108602] ; National Key R&D program of China[2017YFC0108605] ; National Nature Science Foundation of China[11805246] ; National Nature Science Foundation of China[31400723] ; Hundred-Talent Program of Chinese Academy of Sciences[Y763050BRO] ; Science and Technology Research Project of Gansu Province[145RTSA012] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000512898800022 |
| 出版者 | IVYSPRING INT PUBL |
| 资助机构 | National Key R&D program of China ; National Nature Science Foundation of China ; Hundred-Talent Program of Chinese Academy of Sciences ; Science and Technology Research Project of Gansu Province |
| 源URL | [http://119.78.100.186/handle/113462/141666]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Wang, Jufang |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China 4.Soochow Univ, Med Coll, Suzhou 215123, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Nan,Hua, Junrui,He, Jinpeng,et al. The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b[J]. JOURNAL OF CANCER,2020,11(8):2222-2233. |
| APA | Ding, Nan.,Hua, Junrui.,He, Jinpeng.,Lu, Dong.,Wei, Wenjun.,...&Wang, Jufang.(2020).The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b.JOURNAL OF CANCER,11(8),2222-2233. |
| MLA | Ding, Nan,et al."The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b".JOURNAL OF CANCER 11.8(2020):2222-2233. |
入库方式： OAI收割
来源：近代物理研究所
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