Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish
文献类型：期刊论文
| 作者 | Ma, Jianlong1,2,3; Si, Tiantian1,2,3; Yan, Chaoxian4; Li, Yijing1,2; Li, Qiang5 ; Lu, Xiaofeng1,2; Guo, Yong1,2
|
| 刊名 | ACS SENSORS
 |
| 出版日期 | 2020 |
| 卷号 | 5期号:1页码:83-92 |
| 关键词 | fluorescence probes near-infrared zebrafish bioimaging in situ acetylcholinesterase |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.9b01717 |
| 通讯作者 | Guo, Yong(guoyong@licp.cas.cn) |
| 英文摘要 | Acetylcholinesterase (AChE) plays crucial roles in numerous physiological processes such as cell differentiation, cell apoptosis, and nerve tissue developments. Hence, it is highly necessary to design a fluorescent probe for monitoring AChE activity in complex living organisms. In this work, a near-infrared (NIR) off-on probe (CyN) was developed for AChE detection. CyN was exactly synthesized by introducing an N,N-dimethyl carbamyl moiety to hemicyanine (CyOH). AChE can "light up" strong NIR fluorescence through a cleavage special ester bond and transform CyN into CyOH. Moreover, CyN was qualified for imaging the dynamic change of AChE activity in PC12 cells with retinoic acid or hypoxia stimulation. In particular, the probe has been successfully applied for in situ tracing the intact distribution of AChE in living zebrafish. The observations indicate that major occurrence sites of endogenic AChE on zebrafish are the yolk sac and neuromasts. Overall, CyN shows great potential for use in AChE-related physiological studies. |
| WOS关键词 | TURN-ON ASSAY ; FLUOROMETRIC ASSAY ; HYPOCHLOROUS ACID ; PERIPHERAL SITE ; LATERAL-LINE ; EXPRESSION ; SENSOR ; BUTYRYLCHOLINESTERASE ; VISUALIZATION ; PRECURSORS |
| 资助项目 | National Natural Science Foundation of China[21575149] ; National Natural Science Foundation of China[21505146] ; National Natural Science Foundation of China[21575148] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000510079300013 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://119.78.100.186/handle/113462/141669]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Guo, Yong |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Jianlong,Si, Tiantian,Yan, Chaoxian,et al. Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish[J]. ACS SENSORS,2020,5(1):83-92. |
| APA | Ma, Jianlong.,Si, Tiantian.,Yan, Chaoxian.,Li, Yijing.,Li, Qiang.,...&Guo, Yong.(2020).Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.ACS SENSORS,5(1),83-92. |
| MLA | Ma, Jianlong,et al."Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish".ACS SENSORS 5.1(2020):83-92. |
入库方式： OAI收割
来源：近代物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。