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Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy

文献类型：期刊论文

作者Ma, Chen1,2; Zhang, Ludi2,3; He, Ting4; Cao, Huiying1,2; Ren, Xiongzhao5; Ma, Chenhui1,2; Yang, Jiale1,2; Huang, Ruimin1,2; Pan, Guoyu1,2
刊名STEM CELL RESEARCH & THERAPY
出版日期2021-10-30
卷号12期号:1页码:12
关键词Raman spectroscopy Cell therapy Identification Proliferating human hepatocytes Dedifferentiation
DOI10.1186/s13287-021-02619-9
通讯作者Huang, Ruimin(rmhuang@simm.ac.cn) ; Pan, Guoyu(gypan@simm.accn)
英文摘要Background Cell therapy provides hope for treatment of advanced liver failure. Proliferating human hepatocytes (ProliHHs) were derived from primary human hepatocytes (PHH) and as potential alternative for cell therapy in liver diseases. Due to the continuous decline of mature hepatic genes and increase of progenitor like genes during ProliHHs expanding, it is challenge to monitor the critical changes of the whole process. Raman microspectroscopy is a noninvasive, label free analytical technique with high sensitivity capacity. In this study, we evaluated the potential and feasibility to identify ProliHHs from PHH with Raman spectroscopy. Methods Raman spectra were collected at least 600 single spectrum for PHH and ProliHHs at different stages (Passage 1 to Passage 4). Linear discriminant analysis and a two-layer machine learning model were used to analyze the Raman spectroscopy data. Significant differences in Raman bands were validated by the associated conventional kits. Results Linear discriminant analysis successfully classified ProliHHs at different stages and PHH. A two-layer machine learning model was established and the overall accuracy was at 84.6%. Significant differences in Raman bands have been found within different ProliHHs cell groups, especially changes at 1003 cm(-1), 1206 cm(-1) and 1440 cm(-1). These changes were linked with reactive oxygen species, hydroxyproline and triglyceride levels in ProliHHs, and the hypothesis were consistent with the corresponding assay results. Conclusions In brief, Raman spectroscopy was successfully employed to identify different stages of ProliHHs during dedifferentiation process. The approach can simultaneously trace multiple changes of cellular components from somatic cells to progenitor cells.
WOS关键词STEM-CELLS ; LIVER ; MICROSPECTROSCOPY
资助项目'Organ Reconstruction and Manufacturing' Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020205] ; National Science Foundation of China[81872927] ; National Science Foundation of China[81771890] ; National Science Foundation of China[91859106] ; International Partnership Program of Chinese Academy of Sciences[153631KYSB20160004] ; Independent Deployment Program of the Institute of Pharmaceutical Innovation of the Chinese Academy of Sciences[CASIMM0120184005] ; Shanghai Science and Technology Committee[20S11901400] ; Shanghai Municipal Science and Technology Major Project
WOS研究方向Cell Biology ; Research & Experimental Medicine
语种英语
出版者BMC
WOS记录号WOS:000712980600009
源URL[http://119.78.100.183/handle/2S10ELR8/298487]  
专题中国科学院上海药物研究所
通讯作者Huang, Ruimin; Pan, Guoyu
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai, Peoples R China
4.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing, Peoples R China
5.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Inst Biochem & Cell Biol,State Key Lab Cell Biol, Shanghai 200031, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Chen,Zhang, Ludi,He, Ting,et al. Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy[J]. STEM CELL RESEARCH & THERAPY,2021,12(1):12.
APA Ma, Chen.,Zhang, Ludi.,He, Ting.,Cao, Huiying.,Ren, Xiongzhao.,...&Pan, Guoyu.(2021).Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy.STEM CELL RESEARCH & THERAPY,12(1),12.
MLA Ma, Chen,et al."Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy".STEM CELL RESEARCH & THERAPY 12.1(2021):12.

入库方式： OAI收割

来源：上海药物研究所

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