A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies
文献类型：期刊论文
| 作者 | Song, Yakai2; Deng, Xiaojie2; Shi, Wei1; Tang, Feng1; Huang, Wei1; Gong, Likun2 ; Qin, Qiuping2
|
| 刊名 | JOURNAL OF IMMUNOLOGICAL METHODS
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 499页码:6 |
| ISSN号 | 0022-1759 |
| 关键词 | FcRn TR-FRET mAb ADC Pharmacokinetics |
| DOI | 10.1016/j.jim.2021.113180 |
| 通讯作者 | Gong, Likun(lkgong@cdser.simm.ac.cn) ; Qin, Qiuping(qpqin@cdser.simm.ac.cn) |
| 英文摘要 | We aimed to develop a homogeneous time-resolved fluorometric energy transfer assay for assessment of human neonatal Fc receptor binding activity with IgG-type antibodies. The assay was configured with FcRn-coupled with Eu cryptate via biotin and streptavidin interaction as donor and IgG1 labeled with d2 as acceptor. Only a single incubation step was involved and no wash step was required. The assay demonstrated good accuracy, precision, linearity and specificity. Our further investigation with a rat pharmacokinetics study revealed that the terminal t1/2 for Trastuzumab and its related three ADCs agreed with the EC50 data. The assay can be applied to various IgGs with modifications to identify antibodies with appropriate binding ability to human FcRn. |
| 资助项目 | National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program' of China[2019ZX09732002-013] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000720364300009 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298952]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Gong, Likun; Qin, Qiuping |
| 作者单位 | 1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Ctr Biotherapeut Discovery Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Dept Immunoassay & Immunochem, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yakai,Deng, Xiaojie,Shi, Wei,et al. A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2021,499:6. |
| APA | Song, Yakai.,Deng, Xiaojie.,Shi, Wei.,Tang, Feng.,Huang, Wei.,...&Qin, Qiuping.(2021).A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies.JOURNAL OF IMMUNOLOGICAL METHODS,499,6. |
| MLA | Song, Yakai,et al."A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies".JOURNAL OF IMMUNOLOGICAL METHODS 499(2021):6. |
入库方式： OAI收割
来源：上海药物研究所
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